痛转化中外周蛋白激酶Cε通路研究进展及电针干预的可能性  

Advances of researches on peripheral PKCε pathway during transformation from acute to chronic pain and possibility of application of electroacupuncture intervention

作　　者：孙海榉 王思思 李晓宇 杜俊英[1] 方剑乔[1] 房军帆[1] SUN Hai-ju;WANG Si-si;LI Xiao-yu;DU Jun-ying;FANG Jian-qiao;FANG Jun-fan(The Third Clinical Medical College,Zhejiang Chinese Medical University;Zhejiang Chinese Medical University, Hangzhou 310053. China)

机构地区：[1]浙江中医药大学第三临床医学院

出　　处：《针刺研究》2019年第7期543-546,F0003,共5页Acupuncture Research

基　　金：国家自然科学基金青年项目(No.81603692);国家自然科学基金面上项目(No.81473772);浙江省医药卫生科技计划(一般项目,No.2016KYA154);2018年浙江省大学生科技创新活动暨新苗人才计划资助项目(No.2018R410055)

摘　　要：蛋白激酶Cε(PKCε)是细胞内重要的信号转导物质之一。本文对PKCε在急性痛向慢性痛转化中的实验研究进展进行了综述。前列腺素E2(PGE2)/EP1受体-Gq-PKCε是外周背根神经节(DRG)中调节慢性疼痛的重要信号通路,并且在急性痛向慢性痛转化的后期也发挥作用。DRG神经元中的PKCε分别通过过表达瞬时受体电位(TRP)香草酸亚型1(TRPV1)和锚蛋白1(TRPA1)诱导机械和热超敏反应,介导急性痛向慢性痛的转化。PGE2诱发的EP1-Gq-PKCε信号传导可能是调节下游TRPA1和TRPV1启动疼痛转化过程的关键环节。近几十年来,电针(EA)已经被用于治疗各类急慢性疼痛,疗效肯定,并能明显抑制DRG神经元中PKCε及其上下游物质的表达。因此推断,EA可能通过干预DRG中的PKCε通路在急慢性痛转化中起作用。本文从痛转化概念、DRG中PKCε信号通路在痛转化中的作用及其在EA干预中的作用机制等方面进行综述,为慢性疼痛的治疗提供新思路。Protein kinase Cε(PKCε)is a transforming oncogene and plays an important role in many cellular processing.In the present paper,we review the development of experimental researches on the acute-chronic pain transformation.Results indicated that prostaglandin E2(PGE2)/EP1 receptor-Gq-PKCε is an important signaling pathway to modulate chronic pain in peripheral dorsal root ganglion(DRG)neurons,and also plays a role in the later stage of hyperalgesia during transformation from acute to chronic pain.PKCεin DRG neurons induces mechanical and thermal hypersensitivity respectively by over expression of transient receptor potential vanilloid 1(TRPV1)and TRP ankyrin-1(TRPA1),further mediating the transformation from acute to chronic pain.Whereas,PGE2-evoked activation of EP1-Gq-PKCεsignaling may be the key link in initiating the pain translation process through regulating downstream TRPA1 and TRPV1.Electroacupuncture(EA)has been used to effectively relieving various types of acute and chronic pain for decades,and can significantly inhibit the expression of PKCεand its upstream and downstream molecules.Therefore,it can be inferred that there exists a possibility of EA interventions in interfering the transformation from acute to chronic pain by regulating peripheral PKCε signaling pathway.

关 键 词：急慢性痛转化 痛觉敏化诱发 蛋白激酶CΕ 电针 

分 类 号：R245.97[医药卫生—针灸推拿学]