基于通路分析剖析水稻农艺性状配合力和杂种优势  

Genetic dissection of combining ability and heterosis of rice agronomic traits based on pathway analysis

作　　者：向丽媛 徐凯[1] 苏静 吴超[1] 袁雄[1] 郑兴飞[2] 刁英[2] 胡中立[2] 李兰芝[1] XIANG Li-Yuan;XU Kai;SU Jing;WU Chao;YUAN Xiong;ZHENG Xing-Fei;DIAO Ying;HU Zhong-Li;LI Lan-Zhi(Hunan Engineering & Technology Research Center for Agricultural Big Data Analysis & Decision-making/Hunan Agricultural University,Changsha 410128,Hunan,China;State Key Laboratory of Hybrid Rice/Wuhan University,Wuhan 430072,Hubei,China)

机构地区：[1]湖南农业大学/湖南省农业大数据分析与决策工程技术研究中心,湖南长沙410128 [2]武汉大学/杂交水稻国家重点实验室,湖北武汉430072

出　　处：《作物学报》2019年第9期1319-1326,I0030-I0056,共35页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2016YFD0100101);湖南省自然科学基金项目(2016JJ3070);湖南农大作物种质创新与资源利用国家重点实验室培育基地开放课题(16KFXM03)资助~~

摘　　要：按照NCII 遗传交配设计构建测交群体,考察包括产量性状在内的9个农艺性状,对水稻农艺性状表型、配合力和杂种优势进行通路分析,以期为水稻品种的培育和改良提供理论基础。结果表明,主穗实粒数与生物调控、千粒重与半胱氨酸和蛋氨酸代谢、主穗长与SNARE 相关囊泡运动、主穗一/二次枝梗与依赖DNA 的转录、株高与大分子代谢过程、主穗颖花数与花粉识别、有效穗数与水解酶活性、单株实粒重与嘌呤核苷结合等通路相关。为获得优良性状,高配合力,杂种优势可以从某一性状的相关通路进行研究,找到调控该通路的相关基因,以期为改良水稻一般配合力和获得杂种优势提供一定帮助。In order to provide theoretical foundation of cultivating and improving rice,under North Carolina design II,we constructed test cross population and conducted pathway analysis of phenotype performance,combining ability and heterosis of agronomic trait in present study.The experiments’ results reveal that the filled grains per panicle,1000-grain weight,main panicle length,primary/secondary branch of main panicle,plant height,spikelet per panicle,tillers per plant,and yield per plant were separately related to pathway of biological regulation,cysteine and methionine metabolism,snare interactions in vesicular transport,DNA-dependent transcription,macromolecular metabolism,recognition of pollen,hydrolase activity,purine nucleoside binding and so on.Therefore,in order to obtain elite hybrids with high combining ability and heterosis,it is feasible to start from studying related pathways of a certain trait and related genes regulating the pathway.

关 键 词：杂交水稻 GWAS 通路分析 配合力 杂种优势 

分 类 号：S511[农业科学—作物学]