人良性前列腺增生组织性激素相关受体ERα、ERβ、GPR30、AR的表达及其与前列腺增生的关系  

Expression of hormone related receptors ERα, ERβ, GPR30, AR and its relationship with human benign prostatic hyperplasia

作　　者：褚靖 潘斌[2] 黄鹏玉 叶啸 谢涛 CHU Jing;PAN Bin;HUANG Pengyu;YE Xiao;XIE Tao(1 Zhuhai Hospital Affiliated to Jinan University,Zhuhai 519000,China)

机构地区：[1]暨南大学附属珠海医院,广东珠海519000 [2]暨南大学附属第一医院,510630

出　　处：《山东医药》2019年第22期20-23,共4页Shandong Medical Journal

基　　金：广东省医学科学技术研究基金项目(B2016138)

摘　　要：目的探究雌激素受体α(ERα)、雌激素受体β(ERβ)、G蛋白偶联雌激素受体30(GPR30)及雄激素受体(AR)在人良性前列腺增生组织的表达及其意义。方法取良性前列腺增生组织标本48例及膀胱癌根治术病理诊断为正常前列腺组织标本19例,利用RT-PCR及Western blotting检测两种前列腺组织中ERα、ERβ、GPR30、ARmRNA及蛋白表达水平。结果与正常前列腺组织比较,良性前列腺增生组织ERα、ARmRNA及蛋白表达明显增加(P均<0.05),GPR30mRNA及蛋白表达明显减少(P均<0.05),ERβmRNA及蛋白表达两者差异无统计学意义(P均>0.05)。结论性激素相关受体表达水平的紊乱可能是诱发良性前列腺增生的危险因素。Objective To explore the expression and significance of estrogen receptorα(ERα),estrogen receptorβ(ERβ),G protein-coupled receptor 30(GPR30)and androgen receptor(AR)in human normal prostate tissues and benign prostatic hyperplasia tissues.Methods The mRNA and protein levels of ERα,ERβ,GPR30,and AR were detected in 48 cases of benign prostatic hyperplasia tissues and 19 cases of normal prostate tissues by RT-PCR and Western blotting.Results Compared with the normal prostate tissues,the mRNA and protein levels of ERαand AR increased significantly(both P<0.05),and the mRNA and protein levels of GPR30 decreased significantly in the benign prostatic hyperplasia tissues(both P<0.05),while no significant difference was found in the ERβmRNA and protein expression between the normal prostate tissues and benign prostatic hyperplasia(both P>0.05).Conclusion The imbalanced expression of estrogen and androgen receptors may be the risk factor of benign prostatic hyperplasia.

关 键 词：良性前列腺增生 雌激素受体α 雌激素受体Β G蛋白偶联雌激素受体30 雄激素受体 

分 类 号：R697.32[医药卫生—泌尿科学]