制马钱子及其制剂中士的宁和马钱子碱的含量测定  

Determination of Strychnine and Brucine in Processed Strychnine Semen and Its Preparation

作　　者：张云静 张自品 张雪燕 翟佳丽 田蕾 ZHANG Yun-jing;ZHANG Zi-pin;ZHANG Xue-yan;ZHAI Jia-li;TIAN Lei(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012;Key Laboratory of Chinese Medicinal Formula of Anhui Province Anhui Hefei 230012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]中药复方安徽省重点实验室,安徽合肥230012

出　　处：《广州化工》2019年第14期121-124,共4页GuangZhou Chemical Industry

基　　金：安徽省自然科学基金项目(1708085QH188);安徽高校自然科学研究项目(KJ2019A0465)

摘　　要：建立了超高效液相色谱(UPLC)测定制马钱子及其复方制剂痹祺胶囊、伸筋丹胶囊和晕复静片中士的宁和马钱子碱含量的方法。采用ZORBAX RRHD Eclipse Plus C18色谱柱;流动相A为甲醇,流动相B为含1.2%乙酸和0.15%三乙胺的水溶液;流动相A-流动相B(24:76,V/V)等度洗脱,流速0.16mL/min;检测波长260nm。士的宁和马钱子碱分别在0.49~19.6μg/mL和0.51~20.4μg/mL的范围内线性良好;加样回收率均大于80.0%,RSD均不超过2.0%。本法准确度高,重复性好,专属性强,适用于制马钱子及其制剂的含量测定。A method for determination of strychnine and brucine in processed strychnine semen and its preparations by ultra-performanceliquid chromatography (UPLC ) was established.ZORBAX RRHDEclipse Plus C 18 columnwas used,mobile phase Awas methanol and mobile phase B was solution of 1.2% acetic acid and 0.15% triethylamine.An isocratic elutionwas carried out using mobile phase A-mobile phase B (24:76,V/V ).The flow rate was 0.16 mL/min,detection wavelength was 260 nm.There was a good linear relationship of strychnine in the range of 0.49~19.6 μg/mL ( R =0.9997 ) and strychnine in the range of 0.51~20.4 μg/mL ( R =0.9998 ).The recoveries were above 80.0%.RSD values were not more than 2.0%.The proposed method was accurate and reproducible,which can be used for the assay of processed strychnine semen and its compound preparation.

关 键 词：制马钱子 士的宁 马钱子碱 超高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]