齐兴肉兔转录组测序及肌肉生长发育相关基因的筛选  

Transcriptome sequencing in Qixing rabbit and gene screening related to muscle growth and development

作　　者：邝良德[1] 雷岷[1] 李丛艳[1] 任永军[1] 郭志强[1] 郑洁[1] 张翔宇[1] 张翠霞[1] 杨超[1] 唐丽[1] 邓小东[1] 谢晓红[1] KUANG Liangde;LEI Min;LI Congyan;REN Yongjun;GUO Zhiqiang;ZHENG Jie;ZHANG Xiangyu;ZHANG Cuixia;YANG Chao;TANG Li;DENG Xiaodong;XIE Xiaohong(Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy,Chengdu 610066,China)

机构地区：[1]四川省畜牧科学研究院动物遗传育种四川省重点实验室

出　　处：《黑龙江畜牧兽医》2019年第14期143-148,178,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：四川省应用基础研究项目(2018JY0265);国家兔产业技术体系项目(CARS-43-D-3);四川省畜牧科学研究院基本科研项目(SASA2017A04);四川省“十三五”畜禽育种攻关项目(2016NYZ0046);四川省财政运行专项(SASA2014CZYX005);四川省农业科技支撑计划项目(2016NZ0002)

摘　　要：为了研究不同发育阶段齐兴肉兔肌肉生长发育相关的差异表达基因,试验选取胚胎期18日龄、26日龄的齐兴肉兔骨骼肌进行转录组测序,与兔基因组数据库进行比对,筛选差异表达基因并进行GO分析,进一步筛选与肌肉生长发育相关的差异表达基因,并通过qRT-PCR对筛选基因进行验证。结果表明:胚胎期26日龄共获得5320个差异表达基因,其中上调基因2607个、下调基因2713个;KEGG功能注释发现差异基因显著富集到癌症通路、细胞骨架调控通路、pi3k-akt信号通路和RAP1信号通路;筛选获得的6个基因与肌肉生长发育关系较为密切,其中上调基因分别为TPM1、CASQ1、MYOZ1、CKM、MYL3基因,下调基因为IGF2BP1基因。In order to study the differential expression genes related to muscle growth and development in different developmental stages of Qixing rabbits, the Qixing rabbits of 18 and 26 days of the embryonic age were selected in this experiment for skeletal muscle transcriptome sequencing. Compared with the rabbit genome database, the differential expression genes were screened, and the GO analysis was carried out for the further screening of the differential expression genes related to muscle growth and development and qRT-PCR was performed for the validation of the selected genes. The results showed that 5 320 differential expression genes were obtained between 26 days and 18 days of the embryonic age,which contained 2 607 up-regulated genes and 2 713 down-regulated genes. KEGG functional annotation revealed that the differential expression genes were significantly enriched in cancer pathway, cytoskeleton regulatory pathway,pi3k-akt signaling pathway and RAP1 signaling pathway. Six screened genes were obtained,which were closely related to muscle growth and development. Among them, the up-regulated genes were TPM1,CASQ1,MYOZ1,CKM and MYL3, the down-regulated gene was IGF2BP1.

关 键 词：齐兴肉兔 肌肉 转录组测序 生长发育 差异表达基因 

分 类 号：Q754[生物学—分子生物学]