矽肺患者血清甘露聚糖结合凝集素与Th17/Treg关系  

Relationship between serum mannose-binding lectin and Th17/Treg cells in patients with silicosis

作　　者：赵娜[1] 吴洁[1,2] 吴奇峰 王海兰[1] 李聪[1] 陈政良[3] ZHAO Na;WU Jie;WU Qifeng;WANG Hailan;LI Cong;CHEN Zhengliang(Guangdong Province Hospital for Occupational Disease Prevention and Treatment,Guangdong Provincial Key Laboratory of Occupational Diseases Prevention and Treatment, Guangzhou, Guangdong 510300, China)

机构地区：[1]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300 [2]峨眉山市疾病预防控制中心,四川峨眉山614299 [3]南方医科大学,510515

出　　处：《中国职业医学》2019年第3期263-268,共6页China Occupational Medicine

基　　金：国家自然科学基金(81771771);国家临床重点专科建设项目(2011-09);广东省职业病防治重点实验室(2017B030314152);广东省自然科学基金(2018A030313569);广东省科技计划项目(2014A020212551);广东省医学科研基金(A2019276)

摘　　要：目的探讨矽肺患者血清甘露聚糖结合凝集素(MBL)与辅助性T淋巴细胞17(Th17)/调节性T淋巴细胞(Treg)细胞平衡关系。方法采用横断面调查方法,以101例男性矽肺患者为矽肺组,62名健康人员为对照组。采用酶联免疫吸附实验检测2组人群血清MBL水平,采用流式细胞术检测周围血Th17、Treg细胞比率,采用实时定量聚合酶链式反应检测周围血单个核细胞中孤独核受体γt(RORγt)和叉头样转录因子3(Foxp3) mRNA的相对表达水平。结果矽肺组患者血清MBL水平高于对照组(P <0. 01);与对照组比较,矽肺患者周围血中的Th17细胞比率和RORγt mRNA相对表达水平均升高(P <0. 05),Treg细胞比率和Foxp3 mRNA相对表达水平均下降(P <0. 05)。矽肺壹期、贰期患者的血清MBL水平与第1秒用力呼气容积、用力肺活量和最大呼气中期流量均呈正相关(P <0. 05)。矽肺患者血清MBL水平与Th17细胞比率、RORγt mRNA相对表达水平均呈负相关(P <0. 05),与Treg细胞比率、Foxp3 mRNA相对表达水平均呈正相关(P<0.05)。结论 MBL可能通过调节Th17/Treg平衡,参与矽肺的发生发展过程。Objective To explore the relationship between serum mannose-binding lectin( MBL) and T helper cell 17( Th17)/regulatory T cells( Treg) balance in patients with silicosis. Methods A total of 101 male patients with silicosis were selected in silicosis group and 62 health individuals in control group using the cross-sectional study. The level of serum MBL was measured by enzyme linked immunosorbent assay. The ratio of Th17/Treg was recorded by flow cytometry.The relative expression of retinoid-related orphan nuclear receptor γt( RORγt) and forkhead box 3( Foxp3) mRNA in peripheral blood mononuclear cell were tested by real-time polymerase chain reaction method. Results The level of serum MBL in silicosis group was higher than that of control group( P < 0. 01). The ratio of Th17 cells and the relative expression of RORγt mRNA increased in silicosis group( P < 0. 05),while the ratio of Treg cells and the relative expression of Foxp3 mRNA decreased in silicosis group( P < 0. 05) compared to the control group. The level of serum MBL had negative correlation with forced expiratory volume in the first second,forced vital capacity and forced expiratory flow( P < 0. 05) in patients with stage Ⅰ and Ⅱ silicosis. Meanwhile,the level of serum MBL had negative correlation with Th17 ratio and RORγt mRNA relative expression( P < 0. 05),and positive correlation with Treg ratio and Foxp3 mRNA relative expression( P < 0. 05). Conclusion MBL might participate in the development of silicosis through regulating the balance of Th17/Treg cells.

关 键 词：矽肺 甘露聚糖结合凝集素 TH17/TREG FOXP3 RORΓT 细胞平衡 

分 类 号：R135.4[医药卫生—劳动卫生]