血中3种卤代烃的气相色谱-质谱测定法  

Detecting three halogenated hydrocarbons in blood by gas chromatography-mass spectrometry

作　　者：蔡小璇[1] 戎伟丰[2] 吴邦华[2] 张爱华[2] 钟新光 彭建梅 叶立和 马争- 唐斯[1] CAI Xiaoxuan;RONG Weifeng;WU Banghua;ZHANG Aihua;ZHONG Xinguang;PENG Jianmei;YE Lihe;MA Zheng;TANG Si(Dongguan Prevention and Treatment Center for Occupational Disease, Dongguan, Guangdong 523008, China)

机构地区：[1]东莞市职业病防治中心,广东东莞523008 [2]广东省职业病防治院,广东省职业病防治重点实验室,广州510300

出　　处：《中国职业医学》2019年第3期363-366,370,共5页China Occupational Medicine

基　　金：东莞市社会科技发展(重点)项目(2018507150051626)

摘　　要：目的建立血中二氯甲烷、三氯甲烷、1,2-二氯乙烷的气相色谱-质谱测定方法。方法采用静态顶空分析法,血样中的3种卤代烃经DB-5MS弹性毛细管色谱柱分离,采用气相色谱-质谱联用仪检测。结果血样中二氯甲烷、三氯甲烷、1,2-二氯乙烷在选定的定量范围内线性关系良好,相关系数均大于0.999 8,检出限为0.19~0.28μg/L,定量下限为0.64~0.93μg/L,加标回收率为95.1%~106.6%。批内相对标准偏差(RSD)为2.9%~4.9%,批间RSD为5.0%~7.0%,样品在常温或4℃下可保存3 d,在-8℃及以下可保存7 d。结论本方法灵敏度高、精密度和准确度好、操作简便、干扰少,可作为二氯甲烷、三氯甲烷、1,2-二氯乙烷的检测方法。Objective To establish a method for detecting dichloromethane,trichloromethane and 1,2-dichloroethane in blood by gas chromatography-mass spectrometry. Methods Using static headspace analysis, three halogenated hydrocarbons in blood samples were separated by DB-5 MS elastic capillary column and detected by gas chromatographymass spectrometry. Results There was a good linear relationship in the selected range of dichloromethane,trichloromethane and 1,2-dichloroethane in blood. The linear correlation coefficient was greater than 0. 999 8. The detection limit and the lower limit of quantitation was 0. 19-0. 28 and 0. 64-0. 93 μg/L,respectively. The average recovery rate was 95. 1%-106. 6%. The within-run and between-run relative standard deviation was 2. 9%-4. 9% and 5. 0%-7. 0%,respectively. The samples could be preserved at room temperature or 4 ℃ for 3 days and at-8 ℃ or below for7 days. Conclusion With the features of high sensitivity,precision,accuracy,easy operation and less interference,this method is suitable for detecting dichloromethane,trichloromethane and 1,2-dichloroethane in the blood.

关 键 词：气相色谱-质谱法 血样 顶空分析法 卤代烃 二氯甲烷 三氯甲烷 1 2-二氯乙烷 

分 类 号：R134.4[医药卫生—劳动卫生]