临床标本呼吸道病毒感染检测与腺病毒分离鉴定  

Detection of respiratory viral infection and isolation and identification of human adenovirus

作　　者：曲丽欣 李烨 张哲 董妥 王迎晨[1] 商蕾[1] 高虹[1] O.N.Reva V.I.Zlobin 曲章义[1] QU Li-xin;LI Ye;ZHANG Zhe;DONG Tuo;WANG Ying-chen;SHANG Lei;GAO Hong;Oleg N.Reva;Vladimir Igorevich Zlobin;QU Zhang-yi(Department of Hygienic Microbiology,Public Health College,Harbin Medical University, Harbin 150081,China;Department of Biochemistry,Bioinformatics and Computational University of Pretoria,Pretoria 0002,South Africa;Research Institute for Biomedical Technolo- gies,Irkutsk State Medical University,Irkutsk 664003,Russia)

机构地区：[1]哈尔滨医科大学卫生微生物学教研室,黑龙江哈尔滨150081 [2]比勒陀利亚大学生物信息学与计算生物学部生物化学系,比勒陀利亚南非0002 [3]俄罗斯伊尔库茨克国立医科大学生物医学技术研究所,俄罗斯伊尔库茨克664003

出　　处：《哈尔滨医科大学学报》2019年第2期116-121,共6页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(31370074;81172725;30771909);黑龙江省医学科学院转化专项(201621;201717);哈尔滨医科大学中俄医学中心成果转化专项(人呼吸道腺病毒重组疫苗的转化研究)

摘　　要：目的了解哈尔滨地区住院患者呼吸道病毒感染状况及流行趋势,为疾病防治和腺病毒流行监测提供参考。方法采用直接免疫荧光法,检测2017年3月~2018年2月哈尔滨地区住院患者痰液标本呼吸道病毒的感染情况。选择1例腺病毒阳性标本利用空斑实验进行分离纯化,利用PCR扩增六邻体L1区、纤突和E1A区并进行PCR产物序列测定和Blast比对分析鉴定型别。结果七种常见呼吸道病毒检测总体阳性率为14.36%。呼吸道合胞病毒阳性率最高为4.18%,腺病毒阳性率为1.82%。不同季节病毒检测阳性率差异有统计学意义(P<0.05)。从腺病毒检测阳性标本中分离到一株人腺病毒毒株,进行PCR产物测序和分析,初步鉴定该毒株为人腺病毒3型。结论哈尔滨地区呼吸道病毒感染的病原体主要以呼吸道合胞病毒为主。呼吸道病毒感染流行季节为冬春季节,应加强哈尔滨地区呼吸道病毒流行情况监测。Objective To understand the status and trends of respiratory viral infection in inpatients in Harbin,and provide evidence for disease diagnosis and prevention and adenovirus epidemic surveillance. Methods Direct immunofluorescence was used to detect sputum specimens of inpatients in Harbin from March 2017 to February 2018. One adenovirus positive specimen was isolated and purified by plaque assay. The PCR product was sequenced and the sequence was subjected alignment and BLAST searching in GenBank to identify the exact type of adenovirus. Results Total positive rate of the seven kinds respiratory viruses was 14. 36%. The positive rate of RSV was highest and the positive rate of adenovirus was 1. 82%. The posi- tive rate of virus among different seasons was statistically significant ( P < 0. 05 ). BLAST search analysis on hexon L1 region and the fiber protein genes and E1A region showed that ade- novirus strain isolated was homologous to human adenovirus type 3 strain. Conclusion RSV is the major pathogen of respiratory virus infection in the area of Harbin in 2017. The epidemic seasons of respiratory viral infection are the winter and spring and the monitoring of respiratory virus should be strengthened in Harbin.

关 键 词：呼吸道病毒 直接免疫荧光 腺病毒 病毒培养 

分 类 号：R181.32[医药卫生—流行病学]