兴安升麻根茎的化学成分研究  

Chemical constituents from rhizomes of Cimicifuga dahurica

作　　者：卢青 李海波[2] 姚新生[1] 于洋[1] LU Qing;LI Hai-bo;YAO Xin-sheng;YU Yang(Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Kanion Pharmaceutical Co., Ltd., Lianyungang222001, China)

机构地区：[1]暨南大学中药及天然药物研究所,广东 广州 510632 [2]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,江苏 连云港 222001

出　　处：《中草药》2019年第14期3261-3268,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金重点项目(81602984);国家自然科学基金重点项目(81630097)

摘　　要：目的研究兴安升麻Cimicifuga dahurica根茎的化学成分。方法运用HP-20大孔吸附树脂、硅胶、ODS和Sephadex LH-20等柱色谱以及反相半制备型HPLC等各种现代色谱分离技术进行系统的分离纯化,根据化合物的光谱数据和理化性质进行结构鉴定。结果从兴安升麻根茎大孔吸附树脂30%乙醇水洗脱部位中共分离得到了20个化合物,分别鉴定为cimicifugaside F(1)、(+)-(2S,3R)-2-(4-羟基-3-甲氧基苯基)-3-[(β-D-吡喃葡萄糖氧基)甲基]-7-甲氧基苯并呋喃-5-丙烯酸(2)、5-羟基-2-甲氧基苯甲酸(3)、苯甲酸-4-O-β-D-葡萄糖苷(4)、异阿魏酸(5)、阿魏酸(6)、阿魏酸-4-O-β-D-阿洛糖苷(7)、阿魏酸-4-O-β-D-葡萄糖苷(8)、芥子酸-4-O-β-D-葡萄糖苷(9)、6,6′-二-O-芥子酰基蔗糖(10)、番石榴酸(11)、蜂斗菜酸(12)、N-反式对羟基苯乙基阿魏酰胺-4-O-β-D-阿洛糖苷(13)、N-反式-3′-甲氧基-4′-羟基苯乙基阿魏酰胺-4-O-β-D-阿洛糖苷(14)、N-反式-3′-甲氧基-4′-羟基苯乙基阿魏酰胺-4-O-β-D-葡萄糖苷(15)、grevilloside G(16)、丁香脂素(17)、丁香脂素-4,4′-O-β-D-双阿洛糖苷(18)、(+)-异落叶松树脂素-3a-O-β-D-葡萄糖苷(19)、(-)-5′-甲氧基异落叶松树脂素-3a-O-β-D-葡萄糖苷(20)。结论化合物1为1个新的木脂素苷类化合物,化合物2~4、8~10、15~17、20为首次从升麻属植物中分离得到。Objective To isolate and identify the chemical constituents from the rhizome of Cimicifuga dahurica. Methods The isolation and purification of 60% EtOH extract of the rhizomes of C. dahurica were carried out through various modern chromatographic separation techniques: HP-20, silica gel, ODS, Sephadex LH-20 column and semi-preparative HPLC. And the structures of the compounds were identified based on spectroscopic data and physicochemical properties. Results Twenty compounds were isolated and identified as cimicifugaside F (1),(+)(2S,3R)-2-(4-hydroxy-3-methoxyphenyl)-3-[(β-D-glucopyranosyloxy) methyl]-7-methoxybenzofuran-5-propenoic acid (2), 5-hydroxy-2-methoxybenzoic acid (3), benzoic acid 4-O-β-D-glucoside (4), isoferulic acid (5), ferulic acid (6), trans-ferulic acid 4-O-β-D-allopyranoside (7), trans-ferulic acid 4-O-β-D-glucoside (8),(E)-sinapic acid 4-O-β-D-glucoside (9), 6,6′-di-O-sinapoylsurcose (10), piscidic acid (11), fukinolic acid (12), N-trans-feruloyltyramine 4-O-β-D-allopyranoside (13), N-trans-3′-methoxy-4′-feruloyltyramine-4-O-β-D-allopyranoside (14), N-trans-3′-methoxy-4′- feruloyltyramine-4-O-β-D-glucoside (15), grevilloside G (16),(.)-syringaresinol (17),(.)-syringaresinol 4,4′-di-O-β-Dallopyranoside (18),(+)-isolarisiresinol 3a-O-β-D–glucoside (19),(.)-5′-methoxyisolariciresinol 3a-O-β-D-glucoside (20). Conclusion Compound 1 was identified as a new lignan, and compounds 2-4, 8-10, 15-17 and 20 were isolated from Cimicifuga genus for the first time.

关 键 词：兴安升麻 5-羟基-2-甲氧基苯甲酸 苯甲酸-4-O-β-D-葡萄糖苷 阿魏酸-4-O-β-D-葡萄糖苷 芥子酸-4-O-β-D-葡萄糖苷 丁香脂素 

分 类 号：R284.1[医药卫生—中药学]