杜仲皮化学成分研究  

Chemical constituents from barks of Eucommia ulmoides

作　　者：黄卫星 姚铁[3] 丁丽琴[2] 李巍 HUANG Wei-xing;YAO Tie;DING Li-qin;LI Wei(School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;School of Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China;Faculty of Pharmaceutical Sciences, Toho University, Chiba 274-8510, Japan)

机构地区：[1]天津中医药大学中药学院,天津300193 [2]天津中医药大学中医药研究院,天津300193 [3]沈阳药科大学中药学院,辽宁沈阳110016 [4]日本东邦大学药学部,日本千叶274-8510

出　　处：《中草药》2019年第14期3279-3283,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金重点项目(81430095);国家自然科学青年基金资助项目(81303180)

摘　　要：目的研究杜仲Eucommia ulmoides皮的化学成分。方法采用正相硅胶柱色谱、反向ODS柱色谱、Sephadex LH-20柱色谱、制备HPLC等手段对杜仲皮化学成分进行分离纯化,并根据HR-ESI-MS、NMR等波谱数据进行结构鉴定。结果从杜仲皮95%乙醇提取物中分离得到9个化合物,鉴定为甘草黄酮B(1)、3,5,4′-三羟基-7,3′-二甲氧基黄酮(2)、甘草素(3)、formononetine(4)、(+)-4′-O-methylglabridin(5)、paratocarpin E(6)、龙血素C(7)、threo-1-(4-hydroxy-3-methoxyphenyl)-1-methoxy-2-{4-[1-formyl-(E)-vinyl]-2-methoxyphenoxy}-3-propanol(8)、diospyrosin(9),其中7个黄酮和2个苯丙素类。结论化合物1~9均首次从杜仲属植物中分离得到。Objective To study the chemical constituents of barks of Eucommia ulmoides. Methods Compounds were isolated by means of normal phase silical gel, reverse phase ODS, Sephadex LH-20 column chromatography, and preparative-HPLC, and their structures were identified on the basis of various spectroscopic analyses, including NMR and HR-ESI-MS. Results Nine known compounds, including seven flavonoids and two phenylpropanoids, were isolated from the 95% EtOH extract of barks of E. ulmoides. Their structures were identified as licoflavone B (1), 3,5,4′-trihydroxy-7,3′-dimethoxyflavone (2), liquiritigenin (3), formononetine (4),(+)-4′-O-methylglabridin (5), paratocarpin E (6), loureirin C (7), threo-1-(4-hydroxy-3-methoxyphenyl)-1-methoxy-2-{4-[1-formyl-(E)-vinyl]-2-methoxyphenoxy}-3-propanol (8), and diospyrosin (9). Conclusion Compounds 1-9 are obtained from the genus of Eucommia for the first time.

关 键 词：杜仲 黄酮类 苯丙素类 甘草黄酮B 龙血素C 

分 类 号：R284.1[医药卫生—中药学]