Na2ATP对后肢制动大鼠肌梭胞内游离钙的影响  

Effects of adenosine triphosphate on intracellular free calcium in muscle spindle of hind limb immobilization rats

作　　者：余蕾 赵雪红 樊小力[3] 朱娟霞 李雪萍 YU Lei;ZHAO Xue-hong;FAN Xiao-li;ZHU Juan-xia;Li Xue-ping(Department of Basic Medical,Xi′an Medical University,Xi′an 710021;Medical College,Hubei University ofArts and Science,Xiangyang 441053;Department of Medical,Xi′an Jiaotong University,Xi′an 710016,China)

机构地区：[1]西安医学院基础医学部,西安710021 [2]湖北文理学院医学院,襄阳441053 [3]西安交通大学医学部,西安710016

出　　处：《生物学杂志》2019年第4期47-49,54,共4页Journal of Biology

基　　金：国家自然科学基金青年基金项目(项目编号31300981);陕西省自然科学基础研究计划资助项目(项目批准号2012JM4014)

摘　　要：采用激光扫描共聚焦显微镜技术,观察大鼠后肢制动一周比目鱼肌梭内肌细胞内静息Ca^2+的变化,并以Na2ATP作为对抗措施,观察其对后肢制动大鼠梭内肌细胞Ca^2+的影响。结果显示后肢制动一周,大鼠比目鱼肌梭内肌细胞Ca^2+浓度明显升高,而经Na2ATP注射后,大鼠后肢制动一周梭内肌细胞Ca^2+浓度升高不明显。提示Na2ATP可对抗后肢制动所致的梭内肌细胞钙超载。为大鼠肌梭生理的研究及钙离子与废用性肌萎缩的实验研究提供了有意义的资料。The confocal laser scanning microscopy was adopted to observe the changes of resting Ca^2+ in soleus intrafusal muscle cells of rats after hindlimb immobilization for a week, and the effect of Na 2 ATP on Ca ^2+ cells of intrafusal hindlimb immobilization in rats was observed. The results showed that the resting Ca^2+ in soleus intrafusal muscle cells of rats increased significantly after hindlimb immobilization for a week, and after Na 2 ATP injection, the resting Ca^2+ in soleus intrafusal muscle cells of rats had no obvious increase. The experiment suggests that Na 2 ATP can resist calcium overload of intrafusal muscle cell induced by hindlimb immobilization. This study provides valuable information for the study of rat muscle spindle physiology and the experimental study of calcium ion and weightlessness.

关 键 词：Na2ATP CA^2+ 肌梭 比目鱼肌 

分 类 号：Q445[生物学—生理学]