正交试验、响应面法优化加味三根汤的提取工艺比较  

Comparison of Extraction Process of Jiawei Sangen Decoction Optimized by Orthogonal Experiment and Response Surface Methodology

作　　者：李华妮 郑连营 葛文静 王艳艳[1] 刘长河[1] LI Hua-ni;ZHENG Lian-ying;GE Wen-jing;WANG Yan-yan;LIU Chang-he(Henan Provincial Academy of Traditional Chinese Medicine and Pharmacy,Zhengzhou 450004,China;Henan Children ’s Hospital,Children ’s Hospital Affiliated to Zhengzhou University,Zhengzhou Children ’s Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省中医药研究院,河南郑州450004 [2]河南省儿童医院/郑州大学附属儿童医院/郑州儿童医院,河南郑州450003

出　　处：《中国现代中药》2019年第8期1089-1093,共5页Modern Chinese Medicine

基　　金：河南科技攻关项目(2017ZY0506)

摘　　要：目的:优选加味三根汤水提工艺。方法:以提取总皂苷的含量为指标,采用正交设计法和响应面法对提取次数、提取时间和液料比等条件进行优化,确定最优提取工艺。结果:正交设计法分析的最佳提取工艺为液料比10∶1,提取2次,每次1.5h,总皂苷的质量分数为2.90mg·g^-1;响应面法分析的最优工艺为液料比8∶1,提取2次,每次2h。总皂苷的质量分数为3.21mg·g^-1。结论:两种方法生产耗能基本相同,但响应面法提取的总皂苷含量比正交试验法提高了10.7%,更适合加味三根汤提取工艺。Objective:To optimize water extraction process for the preparation of Jiaweisangen Decoction. Methods:The total saponin was selected as an indicator,the factors including extraction times,extraction time and the liquid to material ratio were investigated by means of orthogonal experiment and response surface methodology,recpectively. Results:The extraction conditions optimized with orthogonal experiment for total saponin was extraction times of two,extraction time of 1.5 h,liquid to material ratio of 10∶ 1 and the total saponin content was 2.90 mg · g^-1 .The optimized process from response surface methodology was extraction times of two,extraction time of 2 h,liquid to material ratio of 8∶ 1 and the total saponin content was 3.21 mg · g^-1 . Conclusion:The two methods consume the same amount of energy,but the total saponin content extracted by the response surface methodology has increased by 10.7 % compared with orthogonal experiment,is more suitable for the extraction process of Jiaweisangen decoction.

关 键 词：加味三根汤 正交试验 响应面法 总皂苷 

分 类 号：R284.2[医药卫生—中药学] R944[医药卫生—中医学]