复方当归注射液对肌腱粘连中成纤维细胞增殖抑制的研究  

The study of inhibitory effect of compound angelica injection on fibroblast proliferation in tendon adhesion

作　　者：王振堂[1] 宝莹娜[2] 李昶[1] 爱新觉罗启承 傅永强 Wang Zhentang;Bao Yingna;Li Chang;Aixinjueluo Qicheng;Fu Yongqiang(Department of Hand and Foot Microsurgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Huhehaote 010030, China;Department of Radiotherapy, Affiliated Hospital of Inner Mongolia Medical University, Huhehaote 010050, China)

机构地区：[1]内蒙古医科大学第二附属医院手足显微外科,呼和浩特010030 [2]内蒙古医科大学附属医院放疗科,呼和浩特010050

出　　处：《中华手外科杂志》2019年第4期293-296,共4页Chinese Journal of Hand Surgery

基　　金：内蒙古自治区自然科学基金项目(2016MS0840).

摘　　要：目的探讨复方当归注射液对人成纤维细胞增殖的抑制作用及机制。方法分别给予人成纤维细胞10、20、40、80mg/ml浓度的复方当归注射液,作用48、72h后利用MTS方法检测其增殖水平变化,并通过ELISA检测了解TGF-β1表达水平,采用实时定量荧光PCR(real-time PCR)检测复方当归注射液作用后TGF-β1的变化水平。结果MTS检测结果显示复方当归注射液作用后,20、40、80mg/ml组的细胞增殖抑制(P<0.05);real-time PCR检测结果显示复方当归注射液作用48h后,成纤维细胞TGF-β1的转录水平与对照组相比,10,20,40m/ml组均降低(P<0.05);ELISA检测结果表明复方当归注射液作用48h后,随着药物浓度的增加,TGF-β1表达水平受到抑制(P<0.05)。结论复方当归注射液能够抑制人成纤维细胞TGF-β1的表达水平,提示其可以抑制人成纤维细胞增殖,并且在作用一定时间后抑制水平呈浓度依赖性,从而可能影响肌腱粘连的发生。Objective To investigate the inhibitory effect and mechanism of compound angelica injection on the proliferation of human fibroblasts. Methods Compound angelica injection with 10, 20, 40 and 80 mg/ml concentrations was administered on human fibroblasts respectively. MTS method was used to detect the proliferation level of human fibroblasts after 48 and 72 hours. The expression level of TGF-β1 was detected by ELISA. Real-time PCR was used to detect the change level of TGF-β1 after compound angelica injection. Results MTS results showed that the proliferation of fibroblasts was inhibited in 20, 40, 80 mg/ml group (P<0.05);real-time PCR results showed that the transcription level of TGF-β1 in fibroblasts decreased in 10, 20, 40 mg/ml group compared with the control group 48 hours after the treatment of compound angelica injection (P<0.05). ELISA results showed that the expression of TGF-β1 was inhibited with the increase of drug concentration 48 hours after the treatment of compound angelica injection (P<0.05). Conclusion Compound angelica injection can inhibit the expression of TGF-β1 in human fibroblasts, suggesting that it can inhibit the proliferation of human fibroblasts, and the inhibition level is concentration-dependent after a certain period of time, which may affect the occurrence of tendon adhesion.

关 键 词：成纤维细胞 当归 肌腱粘连 转化生长因子-Β1 

分 类 号：R285[医药卫生—中药学]