Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline, thermal hydrolysis and ultrasonic pretreatments  

作　　者：Mengli Wang Ruying Li Qing Zhao 

机构地区：[1]School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China

出　　处：《Frontiers of Environmental Science & Engineering》2019年第3期163-172,共10页环境科学与工程前沿（英文）

基　　金：the National Natural Science Foundation of China (Grant No. 21407113);the Tianjin City Science and Technology Support Program Project (No. 16YFZCSF00320);the Key Projects of National Water Pollution Control and Management of China (No. 2015ZX07306001).

摘　　要：Sewage sludge in the wastewater treatment plants contains considerable amount of antibiotic resistance genes (ARGs). A few studies have reported that anaerobic digestion (AD) could successfully remove some ARGs from sewage sludge, but information on the fate of ARGs in sludge pretreatment-AD process is still very limited. In this study, three sludge pretreatment methods, including alkaline, thermal hydrolysis and ultrasonic pretreatments, were compared to investigate the distribution and removal of ARGs in the sludge pretreatment-AD process. Results showed that the ARGs removal efficiency of AD itself was approximately 50.77%, and if these three sludge pretreatments were applied, the total ARGs removal efficiency of the whole pretreatment-AD process could be improved up to 52.50%-75.07%. The ultrasonic pretreatment was more efficient than alkaline and thermal hydrolysis pretreatments. Although thermal hydrolysis reduced ARGs obviously, the total ARGs rebounded considerably after inoculation and were only removed slightly in the subsequent AD process. Furthermore, it was found that the total ARGs concentration significantly correlated with the amount of 16S rRNA gene during the pretreatment and AD processes, and the bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

关 键 词：TETRACYCLINE RESISTANCE GENES SULFONAMIDE RESISTANCE GENES 16S rRNA gene SLUDGE pretreatment ANAEROBIC SLUDGE digestion 

分 类 号：T[一般工业技术]