尖孢镰刀菌古巴专化型MAPK FoSlt2敲除突变体的转录组分析  

Transcriptome analysis of the MAPK FoSlt2 deletion mutant of Fusarium oxysporum f. sp. cubense

作　　者：丁兆建 吴小霞[1] 孙君梅 陈丽霞 傅琪彦 许天委 DING Zhao-Jian;WU Xiao-Xia;SUN Jun-Mei;CHEN Li-Xia;FU Qi-Yan;XU Tian-Wei(Department of Biology, Qiongtai Normal University, Haikou, Hainan 571127, China;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China;Tropical Agricultural Technology College, Hainan College of Vocation and Technique, Haikou, Hainan 570216, China)

机构地区：[1]琼台师范学院生物教研室,海南海口571127 [2]中国热带农业科学院环境与植物保护研究所,海南海口571101 [3]海南职业技术学院热带农业技术学院,海南海口570216

出　　处：《菌物学报》2019年第7期1090-1098,共9页Mycosystema

基　　金：国家自然科学基金(31801690)~~

摘　　要：尖孢镰刀菌古巴专化型Fusarium oxysporum f. sp. cubense(FOC)是威胁香蕉生产的重要土传病原真菌。丝裂原活化蛋白激酶(mitogen-activatedproteinkinase,MAPK)FoSlt2信号通路在调控尖孢镰刀菌古巴专化型的生长发育、细胞壁完整性和致病性方面发挥着重要作用。为了揭示FoSlt2信号通路的致病机理和寻找农药靶标,本研究利用高通量RNA-seq技术对该病菌野生型菌株和FoSlt2敲除突变体菌株的转录组进行了比较分析,结果表明差异表达基因共有2 164个,其中上调表达基因有1 184个,下调表达基因有980个。Gene Ontology(GO)功能分析结果表明,差异表达基因主要参与在结合、催化分子功能组和代谢过程、细胞过程生物学通路中。KEGG功能富集分析结果表明,差异基因主要参与戊糖和葡糖醛酸盐转换、氨基糖和核苷酸糖、氨基葡聚糖降解、磷酸肌醇和碳类物质代谢通路,说明这些通路与尖孢镰刀菌古巴专化型的生长发育和致病性相关。该研究为尖孢镰刀菌古巴专化型致病机制的阐明奠定了理论基础。Fusarium oxysporum f. sp. cubense(FOC) is an important soil-borne pathogenic fungus, which leads to a serious threat to the production of banana in the world. Mitogen-activated protein kinase(MAPK) FoSlt2 signaling pathway plays crucial roles in regulation of growth, cell wall integrity and virulence of FOC. To uncover pathogenesis mechanism of FOC and identify the target of fungicide against FOC, the transcriptome profiles of FOC wild type strain and mutant ΔFoSlt2 were characterized with high-throughput sequencing technology. The data indicated that there were 2 164 differential expressed genes(DEGs) in mutant ΔFoSlt2, of which 1 184 were up-regulated and 980 were down-regulated. The Gene Ontology classification showed that the DEGs were mainly related to binding, catalytic activity, metabolic process and cellular process pathway.The KEGG pathway analysis has also revealed that the DEGs are mainly related to pentose and glucoronate interconversions, amino sugar and nucleotide sugar metabolism, glycosaminoglycan degradation, inositol phosphate metabolism and carbon metabolism pathways. The result indicated that these metabolism pathways were involved in growth and pathogenesis of FOC. This study would provide a basis for illuminating pathogenesis mechanism of FOC.

关 键 词：香蕉枯萎病 尖孢镰刀菌古巴专化型 高通量测序 差异表达基因 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]