熟三七的炮制及其破壁饮片与传统饮片的急性毒性比较  

Comparative Studies on Processing and Acute Toxicity of Ultrafine Granular Power and Decoction Pieces of Panax notoginseng

作　　者：陈勇军 贾世清 杨迎暴 成金乐 CHEN Yongjun;JIA Shiqing;YANG Yingbao;CHENG Jinle(Key Laboratory of Technologies and Applications of Ultrafine Granular Powder of Chisese Materia Medica, State Administration of Traditional Chinese Medicine, Zhongshan, Guangdong 528437, China;Zhongshan Zhongzhi Pharmaceutical Group Co. Lid, Zhongshan, Guangdong 528437, China;Department of Pharmacology,School of Pharmaceutical Sciences,Southern Medical Uniwersity, Guangzhou, Guangdong 510515, China)

机构地区：[1]国家中医药管理局中药破壁饮片技术与应用重点研究室,广东中山528437 [2]中山市中智药业集团有限公司,广东中山528437 [3]南方医科大学药学院药理学系,广东广州510515

出　　处：《今日药学》2019年第7期464-467,共4页Pharmacy Today

摘　　要：目的研究熟三七的炮制工艺,初步评价三七及熟三七破壁饮片与其传统饮片在急性毒性方面的差异。方法通过比较生三七、油炸熟三七、常压蒸制熟三七、高压蒸制熟三七四者含量变化,评价各炮制方法差异;各组动物分别以允许的最大给药剂量灌胃给药,给药后观察动物一般情况,并记录动物的不良反应和死亡情况,连续观察7 d。结果油炸和蒸制时间过长均使含量下降明显;给药后动物除出现活动减少,神情倦怠,进食减少外,无其他明显异常反应,在2 h后基本恢复正常。连续观察7 d未见动物死亡,体质量变化不明显,末次称重后处死小鼠进行解剖观察主要脏器,均无肉眼可见异常。结论熟三七炮制以105℃高压蒸制1.5 h为宜;三七、熟三七破壁饮片及其传统饮片小鼠每天的灌胃最大给药量为生药120 g·kg^-1·d^-1,均无明显毒性反应产生。OBJECTIVE To study the processing methods and processing conditions of cooked Panax notoginseng. Tentatively evaluate the difference in acute toxicity between Panax notoginseng and its cooked products ultrafine granular power of Chinses material medica and their traditional pieces. METHODS Determine the quantity of saponins among raw Panax notoginseng,fried Panax notoginseng,steamed Panax notoginseng,steaming under high pressure Panax notoginseng by HPLC. Animals in each group were intragastrically administered with the maximum allowable dose. Afterthat,the general condition of the animals was observed,and the adverse reactions and deaths of the animals were recorded for 7 days. RESULTS The quantity of saponins significantly reduced after long time cooked. After the administration,besides the animals showed decrease in activity and appetite,there were no other obvious abnormal reactions,and they returned to normal condition after 2 hours. After 7 days of continuous observation,no animal died,and the body weight change was not obvious. The mice were sacrificed after the last weighing and the main organs were dissected and observed.Also,no abnormalities were appeared through anatomical and histological observation. CONCLUSION The temperature 105 ℃ and the time 1. 5 h high pressure steaming can be adapted to prepare cooked Panax notoginseng. The maximum daily dose of Panax notoginseng and its cooked products and their traditional decoction was crude drug 120 g·kg^-1·d^-1,and no obvious toxicity was observed.

关 键 词：熟三七 炮制 传统饮片 破壁饮片 急性毒性 

分 类 号：R285.5[医药卫生—中药学]