帕瑞昔布钠对脓毒症大鼠肠系膜微循环的影响  

Effects of parecoxib on mesenteric microcirculation in a rat model of sepsis

作　　者：刘志慧[1] 于泳浩[2] 李佩铂[1] Liu Zhihui;Yu Yonghao;Li Peibo(Department of Anesthesiology, Baotou Central Hospital, Baotou 014040, China;Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin 300052, China)

机构地区：[1]包头市中心医院麻醉科,014040 [2]天津医科大学总医院麻醉科,300052

出　　处：《国际麻醉学与复苏杂志》2019年第7期617-621,共5页International Journal of Anesthesiology and Resuscitation

基　　金：内蒙古自治区自然科学基金(2017BS0802).

摘　　要：目的观察帕瑞昔布钠对脓毒症大鼠肠系膜微循环的影响。方法将32只Wistar大鼠按随机数字表法分为4组(每组8只):假手术组(Sham组)、假手术+10mg/kg帕瑞昔布钠组(SP组)、脓毒症组[盲肠结扎穿孔(cecal ligation and puncture,CLP)组]、脓毒症+10mg/kg帕瑞昔布钠组(CP组)。利用CLP法建立脓毒症大鼠模型后20min,向SP组和CP组大鼠腹腔注射帕瑞昔布钠,每12h重复注射1次,共2次。给予Sham组和CLP组大鼠腹腔注射等量的生理盐水。大鼠颈动脉进行插管以连续监测基础血流动力学(MAP、心率),于造模后0、6、12h行血气分析,测定小肠肠系膜的微循环变化[总血管密度(total vessel density,TVD)、灌注血管密度(perfusedvesseldensity,PVD)、灌注血管比例(proportion of perfused vessel,PPV)及微血管血流指数(microcirculatory flow index,MFI)]。结果与CLP组比较,CP组在造模后2、4、6、8hMAP明显升高,心率明显降低(P<0.05);CP组在造模后6、12h动脉血pH和PaO2/FiO2明显升高,PaCO2和乳酸水平降低(P<0.05);CP组在造模后6、12、24h肠系膜TVD、PVD、MFI明显升高(P<0.05)。结论帕瑞昔布钠对脓毒症大鼠的肠系膜微循环的改善有显著作用,可增加脓毒症时肠系膜微循环TVD、PVD、MFI。Objective To observe the effects of parecoxib on mesenteric microcirculation in sepsis rats. Methods Thirty-two Wistar rats were randomly assigned into 4 groups (n=8): sham operation group(group Sham), sham+10 mg/kg parecoxib group (group SP), sepsis group [group cecal ligation and puncture procedure (CLP)], CLP+10 mg/kg parecoxib group (group CP). CLP was applied to induce sepsis. Rats were injected parecoxib by intraperitoneal at 20 min after CLP or sham operation, injection was repeated every 12 h, twice in all. Rats in sham group and CLP group were given intraperitioneal injection of the same amount of saline. The changes of hemodynamics and blood gas were measured. Moreover, the mesenteric microcirculation, including perfused vessel density (PVD), total vessel density (TVD), proportion of perfused vessel (PPV) and microcirculation flow index (MFI) of rats with sepsis were detected after CLP or sham operation. Results Compared with CLP group, Parecoxib treatment markedly improve hemodynamics, increasing MAP and reducing heart rate in group CP at 2, 4, 6 h and 8 h after modeling (P<0.05). It can effectively improve the blood gas index of sepsis rats, arterial blood pH and PaO2/FiO2 increased significantly, PaCO2 and lactic acid decreased significantly in group CP at 6, 12 h and 24 h after modeling (P<0.05). Parecoxib treatment markedly improve the mesenteric microcirculation and increase the TVD, PVD and MFI in group CP at 6, 12 h and 24 h after modeling (P<0.05). Conclusions Parecoxib sodium treatment may improve mesenteric microcirculation in septic rats, increase the density of TVD, PVD and MFI.

关 键 词：脓毒症 帕瑞昔布钠 微循环 

分 类 号：R459.7[医药卫生—急诊医学]