低氧运动对营养性肥胖大鼠骨骼肌AMPK-PGC-1α的影响  

Influence of Hypoxia Exercise on AMPK-PGC-1α in the Skeletal Muscle of the Rats with Alimentary Obesity

作　　者：吴菊花[1] 钟红梅[2] 杨亚南 赵芳芳 徐国琴 翁锡全[5] 林文弢 Wu Juhua;Zhong Hongmei;Yang Yanan;Zhao Fangfang;Xu Guoqin;Weng Xiquan;Lin Wentao(College of Physical Education of Guangxi University of Science and Technology,Liuzhou 545006,China;Lushan College of Guangxi University of Science and Technology,Liuzhou 545006,China;Guangdong Provincial Football Center,Guangzhou 510080,China;Zhuhai College of Jilin University,Zhuhai 510500,China;Guangzhou Sport University,Guangzhou 519041,China)

机构地区：[1]广西科技大学体育学院,柳州545006 [2]广西科技大学鹿山学院 [3]广东省足球运动中心 [4]吉林大学珠海学院 [5]广州体育学院

出　　处：《中国运动医学杂志》2019年第6期486-492,共7页Chinese Journal of Sports Medicine

基　　金：教育部人文社会科学研究青年基金西部和边疆项目(18XJCZH009);广西科技大学博士基金(校科博17083);广西科技大学运动与健康实验室科研培育项目(GKDTYSYS005);广西高校中青年教师基础能力提升项目(2018KY0314)

摘　　要：目的:探讨低氧运动对营养性肥胖大鼠骨骼肌AMPK-PGC-1α的影响。方法:构建7周高脂膳食诱导SD大鼠营养性肥胖模型,通过眼眶采血,京都血糖试纸测定血糖(BG),GPO-PAP法测定甘油三酯(TG),COD-PAP法测定总胆固醇(TC),聚乙烯硫酸沉淀法测定低密度脂蛋白(LDL-c);称重,测体长,计算BMI;确认营养性肥胖大鼠模型构建成功。建模后随机分为常氧高脂膳食安静组和运动组(NHQ和NHE组,各10只)、16.3%低氧高脂膳食安静组和运动组(HGQ1和HGE1组,各10只)、13.3%低氧高脂膳食安静组和运动组(HGQ2和HGE2组,各10只),继续高脂饲养,运动组进行8周耐力训练,即20 m/min,40 min/d,5 d/w。末次运动24 h后处死大鼠并采样;Western blotting测定大鼠骨骼细胞中AMPK蛋白、pAMPK蛋白、PGC-1α蛋白表达量。结果:(1)7周高脂膳食可诱导大鼠体重、BMI、BG、TC、LDL-c、TG含量增加(P<0.01或P<0.05)。(2)在大鼠骨骼肌AMPK磷酸化程度方面,HGE1组高于NHQ组(P<0.05),而HGE2组高于NHQ组、NHE组、HGQ1组(P<0.01)。在大鼠骨骼肌PGC-1α蛋白表达方面,HGE2组高于NHQ组(P<0.01),NHE组和HGE1组高于NHQ组(P<0.05);HGE2组高于NHE组(P<0.05);HGE2组和HGE1组均高于HGQ1组(P<0.01或P<0.05)。结论:低氧运动可能通过增强AMPK磷酸化,进而上调PGC-1α蛋白的表达,这可能是低氧运动改善营养性肥胖大鼠骨骼肌脂代谢紊乱的机制之一。Objective To explore the effect of hypoxia exercise on the protein expression of AMP-activated protein kinase(AMPK) peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC-1α) in the skeletal muscle of the rats with alimentary obesity. Methods The alimentary obesity was induced in Sprague-Dawley rats after 7-week high-fat diet. Then the venous blood was collected to measure the blood glucose(BG),triglyceride(TG),total cholesterol(TC) and low-density lipoprotein(LDL-c) using the kyoto-glucose dipstick,GPO-PAP method,COD-PAP method and polyethylene sulfate precipitation method respectively. The weight and body length of rats both were measured,and the BMI was calculated. After the measuring,rats with successful modelling were randomly divided into a nomoxic group(NHQ),a nomoxic group with endurance exercise(NHE),a 16.3% hypoxia group(HGQ1),a 16.3% hypoxia group with endurance exercise(HGE1),a 13.3% hypoxia group(HGQ2)and a 13.3% hypoxia group with endurance exercise(HGE2),each of 10. All rats continued to receive a high-fat diet,while those of the groups with endurance exercise took a 40-minute treadmill running at the speed of 20 m/min every weekday,lasting 8 weeks. Twenty-four hours after the last exercise,all rats were killed and sampled to detect the protein expression of PGC-1α,pAMPK and AMPK using the Western blotting. Results A 7-week high-fat diet increased the body weight, BMI, BG,CHO, LDL-c and TG of rats significantly. The phosphorylation protein expression of AMPK of the HGE1 group was significantly higher than NHQ group(P<0.05). The phosphorylation protein expression of AMPK of the HGE2 group was significantly higher than the NHQ group,NHE group,and HGQ1 group respectively(P<0.01). The protein expression of PGC-1α of the HGE2,HGE1 and NHE group was significanlty higher than the NHQ group(P<0.01 or P<0.05). The protein expression of PGC-1αof the HGE2 group was significantly higher than the NHE group(P<0.05). The protein expression of PGC-1α of the HGE2 group and HGE1 group was sign

关 键 词：低氧运动 营养性肥胖 AMPK PGC-1Α 

分 类 号：G804.2[文化科学—运动人体科学]