H9N2亚型流感病毒野鸟分离株HA受体亲和特性鉴定及基因序列分析  

HA Receptor Affinity Identification and Gene Sequence Analysis of H9N2 Subtype AIV Strains Isolated from Wild Birds

作　　者：刘梦迪 姚贵哲 杨帆 孟庆峰 丛彦龙 王伟利 李玥 LIU Mengdi;YAO Guizhe;YANG Fan;MENG Qingfeng;CONG Yanlong;WANG Weili;LI Yue(College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130000, China;College of Veterinary Medicine, Jilin University, Changchun 130062, China)

机构地区：[1]吉林农业大学动物科学技术学院 [2]吉林出入境检验检疫局 [3]吉林大学动物医学学院

出　　处：《吉林农业大学学报》2019年第4期477-482,共6页Journal of Jilin Agricultural University

基　　金：吉林省重点科技攻关项目(20150204029NY)

摘　　要：为了解H9N2亚型流感毒株的变异及跨种间传播机制,通过基因序列测定和固相酶联试验对2016 2017年吉林地区的8株H9N2亚型流感病毒野鸟分离株的HA氨基酸序列及HA的受体结合特性进行了分析。结果表明:8株病毒均具有与人型受体和禽型受体结合特性,其中3株病毒HA的226位氨基酸为L,病毒则偏嗜结合6,SL即人型受体,其余5株病毒HA的226位氨基酸为Q,病毒偏嗜结合3,SL即禽型受体,这暗示着H9N2亚型病毒存在着感染人的潜能,因此应密切监测野鸟H9N2亚型流感病毒的流行特征,为动物及人间流感的防控提供参考。In order to understand the variation and the mechanism of cross-species transmission mechanism of influenza subtype strains,we analyzed the HA sequence and receptor affinity of 8 H9 N2 strains isolated from wild birds in Jilin province from 2016 to 2017 through gene sequence analysis and ELISA. The results showed that all the 8 strains had binding ability to both human type receptor and avian type receptor. Among them,HA 226 amino acid of 3 strains of virus is L,in which case the virus is biased to combine 6’SL-type receptor,namely human receptor. In the remaining 5 strains,HA 226 amino acid is Q,which is biased to bind 3’ SL-bird-type receptor. The results suggests that the influenza virus has the potential to infect human beings,posing the importance of molecular epidemiological investigation of influenza subtype influenza virus and providing the basis for the prevention and control of human influenza.

关 键 词：流感病毒 H9N2亚型 HA 序列分析 受体结合位点 跨种传播 

分 类 号：S855.3[农业科学—临床兽医学] S865.3[农业科学—兽医学]