DNA TyperTM 19试剂盒在欧洲、非洲和东亚人群中的适用性  

Applicability of DNA TyperTM 19 Kit to Distinguish European, African and East Asian Population

作　　者：马泉[1] 江丽[1] 赵慧 黄美莎 邓盼 李玖玲 王玲 马新[1] 叶健[1] 李彩霞[1] MA Quan;JIANG Li;ZHAO Hui;HUANG Meisha;DENG Pan;LI Jiuling;WANG Ling;MA Xin;YE Jian;LI Caixia(Institute of Forensic Science, Ministry of Public Security & Beijing Engineering Research Center of Crime Scene Evidence Examination & National Engineering Laboratory for Forensic Science, Beijing 100038, China;Shanxi Medical University, Taiyuan 030001, China;Kunming Medical University, Kunming 650500, China)

机构地区：[1]公安部物证鉴定中心,北京市现场物证检验工程技术研究中心,现场物证溯源技术国家工程实验室,北京100038 [2]山西医科大学,太原030001 [3]昆明医科大学,昆明650500

出　　处：《刑事技术》2019年第4期327-331,共5页Forensic Science and Technology

基　　金：“十三五”国家重点研发计划(No.2017YFC0803501);国家科技资源共享服务平台计划项目(No.YCZYPT[2017]01-3);中央级公益性科研院所基本科研业务费专项资金项目(No.2015JB023、2016JB039、2017JB025)

摘　　要：目的研究DNATyper^TM19试剂盒的18个STR基因座在欧洲、非洲和东亚人群中的适用性。方法使用DNATyper^TM19复合扩增试剂盒,对欧洲人199份、非洲人188份和东亚人176份无关个体的DNA样本进行扩增,扩增产物用遗传分析仪3130xL进行检测,GenemapperIDv3.2软件对分型结果进行分析。结果在欧洲人中共检测出197种等位基因,等位基因频率分布在0.003~0.568之间;非洲人中共检测出193种等位基因,等位基因频率分布在0.003~0.436之间;东亚人中共检测出187种等位基因,等位基因频率分布在0.003~0.540之间;欧洲人、非洲人与东亚人的累积个体识别能力均大于99.9999999999%。结论DNATyper^TM19复合扩增试剂盒的18个STR基因座在欧洲、非洲、东亚人群中均具有较好的遗传多态性,对三大人群均具有较好的适用性。Objective To investigate the applicability of DNA Typer^TM 19 kit to distinguish European, African and East Asian population. Methods 563 unrelated individuals’ DNA samples, among which 199 pieces are of European, 188 of African and 176 of East Asian, were co-ampli ed with DNA Typer^TM 19 kit to have their 18 STR loci cloned. The ampli ed PCR products were capillary-electrophoresed through 3130XL genetic analyzer, together with their genotyping results being analyzed by Genemapper ID v3.2. Results 197 alleles were identi ed from the European, with their allelic frequencies ranging from 0.003-0.568;193 alleles were found from the African, unveiling their allelic frequencies spanning 0.003-0.436;187 alleles were discovered from the East Asian, bestriding their allelic frequencies over 0.003-0.540. The TDP was greater than 99.999 999 999 9% for all the three populations of European, African and East Asian. Conclusions The DNA Typer^TM 19 kit can be used to analyze genetic polymorphism into European, African and East Asian populations.

关 键 词：法医遗传学 STR 遗传多态性 欧洲人 非洲人 东亚人 

分 类 号：DF795.2[医药卫生—法医学]