三种评估大鼠受孕方法的比较  

Comparison and Analysis of Three Methods to Judge Pregnancy in Rats

作　　者：刘琳[1] 张燕[1] 杜明钰[1] 马润玫[1] LIU Lin;ZHANG Yan;DU Ming-yu;MA Run-mei(Department of Obstetrics,the First Affiliated Hospital of Kunming Medical University,Kunming 650032,Yunnan,China)

机构地区：[1]昆明医科大学第一附属医院产科

出　　处：《医学信息》2019年第16期55-57,共3页Journal of Medical Information

基　　金：国家自然科学基金资助项目（编号：81160082）;昆明医科大学硕士研究生创新基金（编号：2018S016）

摘　　要：目的比较阴栓检查法、阴道涂片法和孕中期腹部触诊法判断SD大鼠受孕的准确性,为高效构建SD孕鼠模型提供参考。方法于2018年1月~3月选取50只未生育过的雌性SPF级SD大鼠,9只SPF级雄性SD大鼠,随机交配后分别用阴栓检查法、阴道涂片法和孕中期腹部触诊法检查大鼠受孕情况,以解剖发现子宫内存在仔鼠为受孕成功的标准,比较三种方法检出大鼠受孕的成功率。结果腹部触诊法判断大鼠受孕的成功率为100.00%,阴道涂片法为90.00%,阴栓检查法为40.00%,差异有统计学意义(P<0.05)。结论孕中期腹部触诊法对确定大鼠受孕成功的准确率最高,但是需结合确定大鼠交配成功准确率较高的阴道涂片法共同判断。Objective To compare the accuracy of pregnancy in SD rats by comparing the method of vaginal suppository examination,vaginal smear and mid-abdominal palpation,and provide reference for the efficient construction of SD pregnant mice.Methods From January to March 2018,50 female SPF SD rats and 9 SPF male Sprague-Dawley rats were randomly divided into two groups.The rats were randomly mated with vaginal smear,vaginal smear and mid-pregnancy.The palpation method was used to check the pregnancy condition of the rats.The sputum was found to be the standard for successful pregnancy in the uterus.The success rate of the conception of the rats was compared by three methods.Results The palpation rate of the abdomen palpation method was 100.00%,the vaginal smear method was 90.00%,and the vaginal suppository method was 40.00%,the difference was statistically significant(P<0.05).Conclusion The palpation method in the second trimester of pregnancy has the highest accuracy in determining the success of pregnancy in rats,but it needs to be judged together with the vaginal smear method that determines the high accuracy of mating success.

关 键 词：阴栓 阴道涂片 腹部触诊 SD大鼠 

分 类 号：Q492.6[生物学—生理学]