鲍曼不动杆菌脓毒症大鼠体内miR-155和 Th17表达的关系  

Expression of microRNA-155 and Th17 in rats with sepsis caused by Acinetobacter baumannii and their relationship

作　　者：张夏子 何先弟[1] 汪华学[1] 邹琪[1] 秦苏徽 ZHANG Xia-zi;HE Xian-di;WANG Hua-xue(Department of Intensive Care Unit,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China)

机构地区：[1]蚌埠医学院第一附属医院重症医学科

出　　处：《中华全科医学》2019年第9期1478-1481,共4页Chinese Journal of General Practice

基　　金：安徽省科技攻关项目(1301042221)

摘　　要：目的观察不同时间段鲍曼不动杆菌脓毒症大鼠血清中miR-155的表达和Th17细胞所占的比例,探究彼此之间关系。方法将32只清洁级SD大鼠按随机数字表法分为正常对照组(N组)、脓毒症6 h组、12 h组和24 h组,每组8只。鲍曼不动杆菌混悬液腹腔注射制作鲍曼不动杆菌脓毒症大鼠模型,对照组腹腔注射生理盐水,外周血分离血清和淋巴细胞,荧光定量RT-PCR法检测大鼠血清中miR-155的表达,流式细胞术检测血液中Th17细胞比例,酶联免疫吸附法(ELISA)测血清白介素17(IL-17)的表达量。分析比较脓毒症6 h组、12 h组、24 h组和正常对照组的组间差异,Pearson相关分析评估miR-155的表达和Th17之间的关系。结果各时间段(6 h、12 h、24 h)脓毒症大鼠miR-155、IL-17和Th17细胞水平较正常对照组升高(均P<0.05)。Pearson相关分析显示血清中miR-155表达与Th17比例水平和IL-17表达均呈正相关(均P<0.05)。结论鲍曼不动杆菌脓毒症大鼠血清miR-155、IL-17和血液中Th17水平与正常对照组之间差异有统计学意义,血清miR-155表达与Th17比例水平和IL-17表达均呈正相关,miR-155有可能会成为鲍曼不动杆菌脓毒症治疗的新靶点。Objective To investigate the percentage of T helper 17(Th17) and miR-155 level in peripheral blood of rats with sepsis caused by Acinetobacter baumannii, and explore their relationships. Methods Total 32 clean SD rats were randomly divided into normal control group(N group), and 6 h, 12 h and 24 h sepsis group, with 8 rats in each group. The sepsis rat model was established by intraperitoneal injection with Acinetobacter baumannii suspension, while the rats in N group were injected with normal saline. The serum and lymphocyte were separated from peripheral blood of rats. The fluorescence quantitative rt-PCR was used to detect expression of miR-155. Flow cytometry was used to detect the ratios of Th17 cells over CD4^+ T cells in peripheral, enzyme-linked immunosorbent assay(ELISA) was used to measure the serum level of interleukin-10(IL-10). The difference among N group and 6 h, 12 h and 24 h sepsis group were analyzed. Pearsons correlation analysis was used to evaluate the relationship between miR-155 and Th17. Results Serum level of miR-155 was significantly higher in rats with sepsis than that in normal group(all P<0.05). Th17 cells was significantly higher in rats with sepsis than that in normal group(all P<0.05). Pearsons correlation analysis showed that the serum level of miR-155 was positively correlated with Th17 cells(all P<0.05). Conclusion The expression of Th17 and miR-155 is increased in Acinetobacter baumannii sepsis rats. The serum level of miR-155 is positively correlated with Th17 cells, which can used as the target in the early diagnosis and treatment of Acinetobacter baumanni.

关 键 词：鲍曼不动杆菌 脓毒症 MIR-155 TH17 

分 类 号：R631[医药卫生—外科学] R378[医药卫生—临床医学]