Effect of the thin cell layer technique in the induction of somatic embryos in Pinus patula Schl. et Cham  

作　　者：Marco A.Ramirez-Mosqueda Lourdes G.Iglesias-Andreu Arturo A.Armas-Silva Esmeralda J.Cruz-Gutierrez Jose F.de la Torre-Sanchez Otto R.Leyva-Ovalle Carlos M.Galan-Paez 

机构地区：[1]Instituto de Biotecnologfa y Ecologfa Aplicada(INBIOTECA),Universidad Veracruzana.Av.de las Culturas Veracruzanas No.101,Campus para la Cultura,las Artes y el Deporte,Col.Emiliano Zapata,C.P.91090 Xalapa,Veracruz,Mexico [2]Centro Nacional de Recursos Geneticos-INIFAP,Boulevard de la Biodiversidad No.400 Rancho las Cruces,C.P.47600 Tepatitlan de Morelos,Jalisco,Mexico [3]Facultad de Ciencias Bioldgicas y Agropecuarias Region Cordoba-Orizaba,Universidad Veracruzana,C.P.94945 Amatlan de los Reyes,Veracruz,Mexico

出　　处：《Journal of Forestry Research》2019年第4期1535-1539,共5页林业研究（英文版）

基　　金：supported by grant from the Mexican Forest Service(CONAFOR)(No.11385232709—“Promotion and Operation of the Forest Genetic Resources Subsystem,within CNRGINIFAP’’)

摘　　要：Pinus patula is a species commonly used for reforestation in Mexico.However,efficient methods for the mass production seedlings are required.Micropropagation particularly by somatic embryogenesis provides an option for the rapid multiplication of high-quality,genetically improved material.This study induces somatic embryogenesis in this species using the thin cell layer(TCL)technique.Two sources of explants(complete immature embryos;lTCL segments from immature embryos)were evaluated.The efficiency of TCL from longitudinal sections[lTCL]and transverse[tTCL]was evaluated.The results show using thin cell layers from immature embryos cultivated in 16 light/8 dark hours achieves induction of somatic embryos.A higher percentage of embryogenic callus was obtained when tTCL segments were used as an explant source.These results produced somatic embryos from tTCL segments of an immature embryo without germinating the seed,making the process more time efficient.In addition,this technique can be used to generate somatic embryogenesis in forest species that have low germination rates.Pinus patula is a species commonly used for reforestation in Mexico.However,efficient methods for the mass production seedlings are required.Micropropagation particularly by somatic embryogenesis provides an option for the rapid multiplication of high-quality,genetically improved material.This study induces somatic embryogenesis in this species using the thin cell layer(TCL)technique.Two sources of explants(complete immature embryos;lTCL segments from immature embryos) were evaluated.The efficiency of TCL from longitudinal sections [lTCL] and transverse [tTCL] was evaluated.The results show using thin cell layers from immature embryos cultivated in 16 light/8 dark hours achieves induction of somatic embryos.A higher percentage of embryogenic callus was obtained when tTCL segments were used as an explant source.These results produced somatic embryos from tTCL segments of an immature embryo without germinating the seed,making the process more time efficient.In addition,this technique can be used to generate somatic embryogenesis in forest species that have low germination rates.

关 键 词：Pinus patula Somatic embryos MICROPROPAGATION THIN CELL LAYER Maturation 

分 类 号：S[农业科学]