原儿茶酸对碱性成纤维细胞生长因子诱导PC12细胞分化的影响  

Influence of Protocatechuic Acid on Differentiation of PC12 Cells in Induced by Basic Fibroblast Growth Factor

作　　者：王丽芳[1] 薛小燕[1] 谢宁生[1] WANG Li-fang;XUE Xiao-yan;XIE Ning-sheng(Ganzhou People's Hospital, Ganzhou Jiangxi 341000, China)

机构地区：[1]赣州市人民医院

出　　处：《抗感染药学》2019年第6期931-933,1008,共4页Anti-infection Pharmacy

基　　金：江西省赣州市指导性科技计划项目(编号:GZ2016ZSF162)

摘　　要：目的:探究原儿茶酸(PCA)对碱性成纤维细胞生长因子(bFGF)诱导PC12细胞分化的影响。方法:体外培养PC12细胞,采用PCA低、中和高剂量组(16.0、32.0和64.0μmol/L)增强bFGF诱导PC12细胞分化,并通过其细胞形态学的观察、细胞活力的检测,分析突起细胞数目及平均突起长度值,考察不同剂量的PCA对bFGF诱导PC12细胞分化的影响。结果:与对照组相比,低、中和高剂量(16.0、32.0和64.0μmol/L)PCA组能增强bFGF诱导PC12细胞分化。结论:PCA能增强bFGF诱导PC12细胞分化的能力。Objective: To explore the influence of protocatechuic acid on cell differentiation of PC12 cells induced by basic fibroblast growth factor (bFGF). Methods: PC12 cells were cultured in vitro, and the bFGF induced PC12 cell differentiation was enhanced at the low-dose, medium-dose and high-dose PCA groups (16.0, 32.0 and 64.0 μmol/L). The number of progenitor cells and the average progenitor length were analyzed through the observation of cell morphology and the detection of cell vitality. The effects of different doses of PCA on the differentiation of bFGF induced PC12 cells were investigated. Results: Compared with the control group, PCA group at low, moderate and high doses (16.0, 32.0 and 64.0 mumol /L) could enhance bFGF induced PC12 cell differentiation. Conclusion: PCA can enhance bFGF's ability to induce PC12 cell differentiation.

关 键 词：原儿茶酸(PCA) 碱性成纤维细胞生长因子(bFGF) PC12 细胞分化 

分 类 号：R285.5[医药卫生—中药学]