地参搽剂的TLC鉴别及梓醇、乙醇的含量测定  

TLC identification and the content determination of catalpol and ethanol in Dishen liniment

作　　者：周许 吴明权 涂禾[1] 张辉武 荣海波[1] 李霞[1] ZHOU Xu;WU Mingquan;TU He;ZHANG Huiwu;RONG Haibo;LI Xia(Department of Traditional Chinese Medicine Research,Sichuan Provincial Orthopedic Hospital,Chengdu,Sichuan ,610041 P. R.China)

机构地区：[1]四川省骨科医院中药研究室

出　　处：《华西药学杂志》2019年第4期379-382,共4页West China Journal of Pharmaceutical Sciences

基　　金：四川省中医药产业创新团队专项(编号:2017C013)

摘　　要：目的建立地参搽剂的质量控制方法。方法采用TLC法对地参搽剂中的苦参、三七和血竭进行定性鉴别;采用HPLC法测定搽剂中梓醇的含量;用GC法测定搽剂中乙醇的浓度。结果TLC鉴别的斑点清晰;梓醇含量测定的线性范围为12.23~611.4μg·mL^-1,平均加样回收率为96.27%,RSD=1.26%(n=6);乙醇含量检测的线性范围为0.1%~1.0%,平均加样回收率为102.9%,RSD=0.70%(n=6)。结论所用方法操作简便,稳定性、重复性均较好,可用于地参搽剂的质量控制。OBJECTIVE To establish a quality control method for Dishen liniment. METHODS Sophorae flavescentis Radix,Notoginseng Radix et Rhizome and Draconis sanguis were qualitatively identified by TLC. The content of catalpol was determined by HPLC and the concentration of ethanol was determined by GC. RESULTS The TLC spots were clear. The catalpol showed good linear relationship at the range of 12. 23 -611.4μg·mL^-1. The average recovery of catalpol was 96. 27% with the RSD of 1.26%( n = 6). The ethanol showed good linear relationship at the range of 0. 1%- 1.0%. The average recovery of ethanol was 102. 9% with the RSD of 0. 70%(n = 6). CONCLUSION The optimized quality control methods are simple, stable and repeatable, and they can be used for the quality control of Dishen liniment.

关 键 词：地参搽剂 苦参 三七 血竭 质量控制 薄层色谱 高效液相色谱 梓醇 气相色谱 乙醇 含量测定 

分 类 号：R917[医药卫生—药物分析学]