亚临界水提取灵芝子实体中灵芝酸类成分的工艺优化  

Optimization of the subcritical water extraction technology of ganoderic acids from the fruiting body of Ganoderma lucidum

作　　者：徐瑞聆 郭思扬 王雅文 杨景淋 傅里馨 丁才航 付春梅[1] XU Ruiling;GUO Siyang;WANG Yawen;YANG Jinglin;FU Lixin;DING Caihang;FU Chunmei(West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P. R. China)

机构地区：[1]四川大学华西药学院

出　　处：《华西药学杂志》2019年第4期393-395,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81473167);四川大学双创专题研究项目(SCUCXCY1754)

摘　　要：目的优化灵芝子实体中灵芝酸类成分的亚临界水提取工艺。方法采用单因素实验设计,HPLC法测定以灵芝酸A和灵芝酸C2为代表的灵芝酸的提取量,考察提取温度、时间、液料比、提取次数对灵芝酸类成分提取量的影响。结果优化的灵芝酸亚临界水提取工艺为:液料比为11,于140℃、5MPa条件下提取5min。结论亚临界水提取法与索氏提取法的提取量相当,较超声提取法高,但耗时更短,且不需要有机溶剂,有利于保护环境。OBJECTIVE To optimize the subcritical water extraction ( SWE) technology of ganoderic acids from the fruiting body of Ganoderma lucidum. METHODS The effect of extraction parameters including temperature, extraction time, the solvent/sample ratio and extraction frequency on the yields were investigated by Single - factor experiment design. The contents of ganoderic acid A and ganoderic acid C2 as the index were analyzed by HPLC. RESULTS The optimum SWE extraction conditions for ganoderic acids were as follows: G. lucidum sample was extracted once at the solvent/sample ratio of 11 under 5 MPa and 140℃ for 5 minutes. CONCLUSION The extraction rate of SWE is equivalent to Soxhlet extraction and better than that of Ultrasonic extraction, but SWE requires less handling time and no organic solvent consumption, which was beneficial to environmental protection.

关 键 词：亚临界水提取法 亚临界水 单因素实验设计 灵芝酸 索氏提取法 超声提取法 绿色化学 

分 类 号：R917[医药卫生—药物分析学]