土贝母苷甲对单纯疱疹病毒1型和2型的增殖和基因组复制的抑制效应  

Inhibitory Effects of Tubeimoside 1 on the Proliferation and Genomic Replication of Herpes Simplex Virus Type 1 and Type 2

作　　者：王巧利 于立坚 王一飞 马润娣 李风 于廷曦 WANG Qiaoli;YU Lijian;WANG Yifei;MA Rundi;LI Feng;YU Tingxi(Guangzhou Jinan Biomedical Research and Development Center Co., Ltd., Guangzhou 510632, China;Department of Product Research and Development, Foshan Depu Biomedical Technology Co., Ltd., Foshan 528231, China;Shanghai Institute for Pediatric Research,Xinhua Hospital, Shanghai Jiao tong University, Shanghai 200092, China)

机构地区：[1]广州暨南生物医药研究开发基地有限公司,广州510632 [2]佛山德溥生物医药科技有限公司产品研发部,佛山528231 [3]上海交通大学新华医院上海市儿科研究所,上海200092

出　　处：《病毒学报》2019年第4期569-577,共9页Chinese Journal of Virology

基　　金：佛山市科技创新专项资金(项目号:2013AA100332);中国科学院南海生物医药科技产业中心支持;教育部重点实验室开放课题(项目号:2016RF01)~~

摘　　要：土贝母苷甲(Tubeimoside 1,TBMS1),简称苷甲,是一种独特的,由贝咢苷元和4种单糖(葡萄糖、鼠李糖、木糖和阿拉伯糖)组成的五环三萜皂苷。以前的研究证实,苷甲具有抗人免疫缺陷病毒的活性。本文研究苷甲对单纯疱疹病毒(Herpes simplex virus,HSV)1型和2型增殖和基因组复制的影响。从土贝母块茎中提取、分离苷甲(在本实验中使用的纯度大于95%),以空斑法测定病毒滴度,通过观察致细胞病变效应和空班形成抑制试验来检测苷甲的抗病毒活性,用实时定量PCR技术检测苷甲对HSV-1和HSV-2基因组复制的影响。结果显示,苷甲显著抑制HSV-1和HSV-2毒株的增殖,显著抑制HSV-1和HSV-2的UL27、UL52和UL54三个基因的拷贝数,且其抑制作用呈明显量效依赖关系。无毒高浓度(0.39μg/mL^0.78μg/mL)的苷甲对阿昔洛韦(acyclovir,ACV)耐药毒株HSV-1/106的增殖也显示抑制活性。本研究结果揭示苷甲具有显著抑制HSV-1和HSV-2增殖及基因组复制的活性,无毒高浓度苷甲对HSV-1/106耐药毒株的增殖也显示抑制活性。Tubeimoside(TBMS)1 is a unique pentacyclic triterpenoid saponin composed of bayogenin and four sugars:glucose,rhamnose,xylose and arabinose. Previous studies have shown that TBMS1 has activity against the human immunodeficiency virus. We investigated the inhibitory effects of TBMS1 on the proliferation and genomic replication of herpes simplex virus type 1(HSV-1)and 2(HSV-2). TBMS1 is extracted and isolated from the tubers of[Bolbostemma paniculatum(Maxim)Franquet(Cucurbitaceae)],and its purity in our study was great than 95%. The toxicity of HSV-1 and HSV-2 was determined by the plaque assay. The antiviral activity of TBMS1 was determined by cytopathic effects and plaque-inhibition test,and quantitative real-time polymerase chain reaction was used to detect the effects of TBMS1 on HSV genomic replication.TBMS1 showed significantly inhibitory effects on the proliferation of HSV-1/F and HSV-2/333 strains,and on copies of UL27,UL52 and UL54 in a dose-dependent manner. TBMS1 of high concentration(0.39 μg/mL^0.78 μ g/mL) was not toxic and showed inhibitory effects on the proliferation of the ACV-resistant strain HSV-1/106. TBMS1 had obvious inhibitory effects on the proliferation and genomic replication of HSV-1 and HSV-2.

关 键 词：土贝母苷甲(TBMS1) 单纯疱疹病毒(HSV)1型和2型 细胞病变效应(CPE) 空班形成抑制试验 增殖 实时定量PCR 基因组复制 

分 类 号：R373.1[医药卫生—病原生物学] R511[医药卫生—基础医学]