冬虫夏草内生菌皮壳青霉菌化学成分的研究  

Chemical constituents of endophytic fungi Penicillium crustosum from Cordyceps Sinensis

作　　者：冯丹[1] 秦玲玲 郑东滨 赵鑫雅 曹雪凤 冉向 戴宇[1] 邓赟[1] FENG Dan;QIN Ling-ling;ZHENG Dong-bing;ZHAO Xin-ya;CAO Xue-feng;RAN Xiang;DAI Yu;DENG Yun(Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,State Key Laboratory Breeding of Systematic Research Development and Utilization of Chinese Medical Resources,Co-founded by Sichuan Province and MOST,Pharmacy Department,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地

出　　处：《中成药》2019年第8期1863-1867,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金面上项目(81373961);四川省科技厅省青年科技创新研究团队专项计划(2016TD0006);四川省教育厅科研项目(18ZA0191);成都中医药大学青年教师创新基金(ZRQN1606)

摘　　要：目的研究冬虫夏草Cordyceps Sinensis内生菌皮壳青霉菌Penicillium crustosum的化学成分。方法冬虫夏草发酵物甲醇提取物的乙酸乙酯部位采用硅胶、Sephadex LH-20、制备型HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到13个化合物,分别鉴定为6-乙酰基-2α,5-二羟基-2-(2-羟丙基)-3α,8-二甲基苯并二氢吡喃(1)、4-甲基-5,6-二氢吡喃酮(2)、3-甲基-4-喹唑啉酮(3)、4-甲氧基苯乙酸(4)、2,4-二甲氧基苯酚(5)、对羟基苯基乙酸甲酯(6)、对羟基苯甲醛(7)、2,5-二羟基-4-甲基苯乙酮(8)、2,4-二羟基-3-甲基苯乙酮(9)、3,4-二羟基苯乙酸甲酯(10)、环(脯氨酸-亮氨酸)(11)、3,4-二甲氧基苯乙醇(12)、3,4-二甲氧基-苯甲酸(13)。结论化合物1~7、9~13为首次从该真菌中分离得到。AIM To study the chemical constituents of endophytic fungi Penicillium crustosum from Cordyceps Sinensis.METHODS The ethyl acetate fraction of methanol extract leavening from Cordyceps Sinensis was isolated and purified by silica, Sephadex LH-20 and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Thirteen compounds were isolated and identified as 6-acetyl-2α,5-dihydroxy-2-(2-hydroxypropyl)-3α,8-dimethylchroman(1), anhydromevalonolactone(2), 3-methylquinazolin-4(3H)-one(3), 2-(4-methoxyphenyl)acetic acid(4), 2,4-dimethoxyphenol(5), methyl 2-(4-hydroxyphenyl)acetate(6), 4-hydroxybenzaldehyde(7), 1-(2,4-dihydroxy-5-methylphenyl)ethan-1-one(8), 1-(2,4-dihydroxy-3-methylphenyl)ethan-1-one(9), methyl 2-(3,4-dihydroxyphenyl)acetate(10), cyclo(Pro-Leu)(11), 3,4-dimethoxy-benzoic acid(12), 2-(3,4-dimeth-oxyphenyl)ethanol(13).CONCLUSION Compounds 1-7 and 9-13 are isolated from this fungus for the first time.

关 键 词：冬虫夏草 内生菌 皮壳青霉菌 化学成分 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]