高通量测序结合可培养技术分析新疆刺腿食蚜蝇内生酵母菌的种群结构  

Analysis of the population structure of endogenous yeast of Ischiodon scutellaris Fabricius in Xinjiang using high throughput sequencing combined with culturing technologies

作　　者：米桃桃 冯丽[1] 王翀[3] 孙燕飞[1] 熊杰[1] 雷勇辉[2] MI Tao-Tao;FENG Li;WANG Chong;SUN Yan-Fei;XIONG Jie;LEI Yong-Hui(College of Life Sciences,Shihezi University,Shihezi 832003,Xinjiang Uygur Autonomous Region,China;Agricultural College of Shihezi University,Shihezi 832003,Xinjiang Uygur Autonomous Region,China;Xinjiang Entry-exit Inspection and Quarantine Bureau,Urumchi 830063,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学农学院,新疆石河子832003 [3]新疆出入境检验检疫局,乌鲁木齐830063

出　　处：《环境昆虫学报》2019年第4期829-836,共8页Journal of Environmental Entomology

基　　金：新疆特色植物基因资源研究与利用创新团队(2014CC005)

摘　　要：对新疆石河子刺腿食蚜蝇 Ischiodon scutellaris Fabricius内生可培养酵母菌和不可培养酵母菌的种类进行鉴定及多样性分析,明确刺腿食蚜蝇主要酵母种类及分布规律,为酵母菌资源的开发利用提供科学依据。通过Illumina (MiSeq)平台高通量测序和生物信息学分析,以及对刺腿食蚜蝇过滤液富集培养,选取其代表菌株进行糖发酵、碳源利用等生理生化检测及26S rDNA D1/D2区进行测序,对刺腿食蚜蝇中可培养酵母菌分离鉴定系统进化分析,得到刺腿食蚜蝇中酵母菌物种分布多样性信息及微生物群落结构组成。从刺腿食蚜蝇体内共分离获得14株可培养酵母菌株,属于 Kodamaea , Saccharomyces , Wickerhamomyces 3个属;不可培养酵母菌含量≥1.49%,主要有16属,为 Filobasidium (黑粉菌属), Udeniomyces , Candida(假丝酵母属), Metschnikowia(梅奇酵母属), Pichia (毕赤酵母属), Prototheca , Papiliotrema , Dipodascus , Kwoniella , Schizosaccharomyces (裂殖酵母), Acaromyces (阿卡酵母属), Cryptococcus (隐球酵母属), Cystofilobasidium , Tetrapisispora , Aureobasidium (金担子菌属), Zygosaccharomyces (接合酵母属)等。研究结果显示刺腿食蚜蝇内生酵母菌组成具有多样性,体内有着丰富的酵母菌种群,需要进一步开展昆虫体内的酵母菌种群的系统研究。To clear and definite the dominant endogenous yeast of Ischiodon scutellaris Fabricius and their distribution in Shihezi, Xinjiang, the diversity of yeast isolated from I.scutellaris were identified and analysed by culture-dependent and culture-independent. It could provided the utilization of yeast resources with scientific basis. By Illumina(MiSeq) high-throughput sequencing and bioinformatics analysis platform, as well as to the I.scutellaris filtrate enrichment culture, selecting the representative strains on sugar fermentation, carbon source utilization of physiological and biochemical tests and 26 s rDNA D1/D2 section sequencing of I.scutellaris in the available to train separation of yeast identification, analysis of system evolution, yeast species distribution in I.scutellaris and their microbial community structure were discovered. A total of 14 culture-dependent yeast strains were isolated from I.scutellaris, including Kodamaea, Saccharomyces, Wickerhamomyces. The ratio over 1.49% among 16 culture-independent yeast genus are as follows: Filobasidium, Udeniomyces, Candida, Metschnikowia, Pichia, Protocheca, Papiliotrema, Dipodascus, Kwoniella, Schizosaccharomyces, Acaromyces, Cryptococcus, Cystofilobasidium, Tetrapisispora, Aureobasidium, Zygosaccharomyces, etc. The results showed that endogenous yeasts isolated from Ischiodon scutellaris Fabricius were abundant and various. It contained abundant yeast populations. Therefore, more systemic researches on endogenous yeast populations isolated from insects were necessary.

关 键 词：刺腿食蚜蝇 内生酵母菌 高通量测序 多样性分析 26SrDNA 

分 类 号：Q965[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]