模板法金纳米簇的制备及其在生物分子检测中的应用  

Preparation of Gold Nanoclusters by Template Method and Applications in Biomolecule Biosensing

作　　者：魏伟 赵倩 石星波 WEI Wei;ZHAO Qian;SHI Xingbo(College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China)

机构地区：[1]湖南农业大学食品科学技术学院

出　　处：《激光生物学报》2019年第4期296-304,共9页Acta Laser Biology Sinica

基　　金：国家自然科学基金青年科学基金项目(31301484);湖南省教育厅项目(18C0131)

摘　　要：金纳米簇(AuNCs)作为一种新型荧光纳米材料,是由几个到约一百个金原子组成的分子聚集体,因制备简单、光学性质优异以及毒性低等特性,近年来在生物传感领域得到了广泛应用。本文首先对以巯基化合物、树枝状化合物、多肽和蛋白质、寡核苷酸DNA等为模板制备AuNCs的模板法及其优点进行阐述,对AuNCs的紫外吸收、荧光及电化学性质进行介绍,之后重点总结基于荧光AuNCs的生物传感器在生物大分子及小分子检测中的应用,最后对AuNCs应用于生物传感领域所面临的挑战进行分析,并对其应用前景进行展望。As a new type of fluorescent nanomaterial, gold nanoclusters (AuNCs) are molecular aggregates composed of several to about one hundred gold atoms. Due to its simple preparation, excellent optical properties and low toxicity, AuNCs have been widely used in the field of biosensing in recent years. This review first briefly introduces the commonly used template method and its advantages for the preparation of AuNCs using sulfhydryl compounds, dendrimer, peptides and proteins, oligonucleotide DNA, etc. as templates. This review describes the UV absorption, fluorescence and electrochemical properties of AuNCs. After that, the application of fluorescent AuNCs-based biosensors in the detection of biomacromolecules and small molecules is summarized. Finally, the challenges of AuNCs in the field of biosensing are analyzed and their applications are prospected.

关 键 词：金纳米簇 荧光 生物传感 生物大分子 小分子 

分 类 号：O614[理学—无机化学] Q503[理学—化学]