基于L-半胱氨酸修饰的Fe3O4@TiO2复合纳米颗粒体外光动力疗法灭活HL60细胞的试验研究  

L-Cysteine Modified Fe3O4@TiO2 Composite Nanoparticles for PDT Inactivation of HL60 Cells in vitro

作　　者：曹林凤 黄铭胜 陈丽[2] 艾保全[1] 熊建文[1] CAO Linfeng;HUANG Mingsheng;CHEN Li;AI Baoquan;XIONG Jianwen(School of Physics and Telecommunication Engineering, South China Normal University,Guangzhou 510006, China;School of Physics and Optoelectronic Engineering,Guangdong University of Technology, Guangzhou 51006, China)

机构地区：[1]华南师范大学物理与电信工程学院,广州510006 [2]广东工业大学物理与光电工程学院,广州510006

出　　处：《激光生物学报》2019年第4期305-313,共9页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(61072029);广州市科技计划资助项目(2014J4100049)

摘　　要：本文采用共沉淀法制备了L-半胱氨酸(L-Cys)修饰的Fe3O4包裹TiO2(Fe3O4@TiO2/L-Cys)复合纳米粒子。通过透射电子显微镜(TEM),X射线衍射(XRD)和傅立叶变换红外光谱仪(FTIR)对复合纳米粒子进行了表征,并讨论了复合纳米粒子对HL60细胞体外光动力疗法(PDT)灭活的影响。并对其PDT灭活机制进行了初步探索。试验表明,Fe3O4@TiO2/L-Cys复合纳米粒子分散性高,生物相容性好,对细胞的暗毒性更低,并可以有效增强靶向性,提高PDT灭活效率,在410nm波长的光激发下,光照剂量为18J/cm^2的情况下,当TiO2与Fe3O4的比例为1∶3时,整体PDT效率最高。PDT灭活效率可达69.36%。In this paper, L-cysteine (L-Cys) modified Fe3O4-coated titanium dioxide (Fe3O4@TiO2/L-Cys) composite nanoparticles were prepared by coprecipitation method.The composite nanoparticles were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The effects of composite nanoparticles on PDT inactivation of HL60 cells in vitro were discussed.The inactivation mechanism of PDT was preliminarily explored. The results showed that Fe3O4@TiO2/L-Cys composite nanoparticles had high dispersibility, good biocompatibility, lower dark toxicity to cells, and could effectively enhance the targeting and improve the inactivation efficiency of PDT. When the irradiation dose was 18 J/cm^2 at 410 nm, the overall PDT efficiency was the highest when the ratio of titanium dioxide to Fe3O4 was 1∶3. The PDT inactivation efficiency of Fe3O4@TiO2/L-Cys was 69.36%.

关 键 词：Fe3O4@TiO2/L-Cys 光动力疗法 HL60细胞 

分 类 号：R454.2[医药卫生—治疗学]