小麦冬前最大分蘖期根数的QTL定位  

QTL Mapping for Root Number of Wheat at Maximum Tillering Stage before Winter

作　　者：彭涛[1,2] 尹国红 成东梅 于金林 赵伟峰 高燕 田纪春 邓志英[1] PENG Tao;YIN Guohong;CHENG Dongmei;YU Jinlin;ZHAO Weifeng;GAO Yan;TIAN Jichun;DENG Zhiying(Shandong Agricultural University/State Key Laboratory of Crop Biology,Tai'an 271018,China;Jiyuan Academy of Agricultural Sciences,Jiyuan 459002,China)

机构地区：[1]山东农业大学/作物生物学国家重点实验室,山东泰安271018 [2]济源市农业科学院,河南济源459002

出　　处：《河南农业科学》2019年第8期27-33,共7页Journal of Henan Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0100500);国家自然科学基金项目(31871613);山东省重点研发计划项目(2017GNC10102);山东省高等学校科技计划项目(J17KA148)

摘　　要：为了研究小麦冬前最大分蘖期根数的分子遗传基础,以小麦加倍单倍体(DH)群体(花培3号×豫麦57)的168个株系为材料,测定3个不同生长环境下DH群体的初生根数、次生根数和总根数,利用已经构建的DH群体遗传连锁图谱,采用基于混合线性模型的复合区间作图法对冬前最大分蘖期根数进行了QTL定位分析。结果表明,共检测到控制初生根数、次生根数、总根数3个性状的7个加性效应QTL和7对上位性互作QTL,分布在1B、2B、2D、3B、4A、4D、5D、6B、6D、7D染色体上,单个QTL可以解释4.67%~16.56%的遗传变异;在1B染色体上的Xwmc406-Xbarc156区间,检测到控制次生根数、总根数的QTL,表现出一因多效或紧密连锁效应,2个主效QTLqSrn1B和qRtn1B分别解释次生根数和总根数表型变异的16.56%和12.80%,可用于小麦根系性状的分子标记辅助选择。In order to study the molecular genetic basis of root number of wheat at maximum tillering stage before winter,168 lines from DH population which was derived from the cross between Huapei No.3 and Yumai 57 were used as materials,based on the established genetic linkage map of DH population,the QTL mapping for the number of primary roots,secondary roots and total roots was conducted using the composite interval mapping method based on mixed linear model under three different environments.The results showed that seven additive QTLs and seven epistatic QTLs controlling the number of primary roots,secondary roots and total roots were detected,which were distributed on 1B,2B,2D,3B,4A,4D,5D,6B,6D and 7D chromosomes.One single QTL could explain 4.67%-16.56% of the phenotypic variation.The QTLs in the marker interval between Xwmc406 and Xbarc156 on 1B chromosome controlled the number of secondary roots and total roots,showing pleiotropism or tight linkage effect.The two major loci, qSrn 1 B and qRtn 1 B ,explained 16.56% and 12.80% of phenotypic variation of secondary roots and total roots number,respectively,which could be used for molecular marker-assisted selection of wheat root traits.

关 键 词：小麦 冬前最大分蘖期 初生根 次生根 DH群体 QTL定位 

分 类 号：S512.1[农业科学—作物学]