百脉根PIN基因家族的鉴定与分析  

Identification and Analysis of PIN Gene Family in Lotus corniculatus

作　　者：吕赟 丘日光 杨仕梅 吴佳海 宋莉 LU Yun;QIU Riguang;YANG Shimei;WU Jiahai;SONG Li(College of Life Sciences and Institute of Agro-Bioengineering/The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)/Guizhou Key Laboratory of Agro-bioengineering,Guizhou University,Guiyang 550025,China;Guizhou Provincial Grass Industry Research Institute,Guiyang 550006,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院/山地植物资源保护与种质创新教育部重点实验室/贵州省农业生物工程重点实验室,贵州贵阳550025 [2]贵州省草业研究所,贵州贵阳550006

出　　处：《河南农业科学》2019年第8期39-48,共10页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31660685);贵州省科技计划项目([2017]1044,[2017]5788,[2016]4024)

摘　　要：生长素输出载体蛋白PIN-formed(PIN)是一类调控植物生长素极性运输的重要载体元件,与植物生长发育密切相关。利用生物信息学方法对百脉根(Lotuscorniculatus)PIN基因家族成员(LjPIN)进行筛选、鉴定,并对其结构特征、系统进化、编码蛋白质性质、顺式作用元件组成和表达特性等进行研究,为其功能鉴定及利用奠定基础。结果表明,百脉根基因组中含有27个LjPIN成员,主要分布在1-5号染色体上;27个LjPIN基因含8个可变剪切位点,编码35个蛋白质;除了LjPIN4、LjPIN13、LjPIN23基因外,其他LjPIN基因均含有内含子,内含子数和外显子数均为1~10个;LjPIN与拟南芥AtPIN、大豆GmPIN在进化关系上较接近,与水稻OsPIN较远;LjPIN蛋白大多为碱性的两性稳定蛋白质,一般含有5种保守基序,基序数量2~9个;LjPIN基因除了含有顺式作用基本元件CAAT-box和TATA-box外,还含有光响应、激素响应及生长发育相关的调控元件;11个LjPIN基因在根、茎、叶、花和不同形成时期种子中呈时空差异表达。PIN-formed protein (PIN) is an important carrier regulating the auxin's polar transport,and is closely related to the plant growth and development.In this study,the PIN family genes ( LjPIN ) were identified from the genetics databases of Lotus corniculatus.These genes were further analyzed for the gene construction,evolution relationship,protein features,cis-acting element site and tissue expression characteristics,etc..Results showed that 27 LjPIN gene members encoding 35 proteins by eight alternative splicing sites were found mainly in chromosomes 1-5.Most genes were made up of 1-10 introns and exons except for the LjPIN4,LjPIN13 and LjPIN23.Evolutionary relationship of LjPIN with Arabidopsis AtPIN and soybean GmPIN was closer than with rice OsPIN.Most LjPIN proteins with 5 kinds of conserved motifs (its number was 2-9) were alkaline and stable.The genes not only included cis-acting elements of CAAT-box and TATA-box,but also contained light-responsive,hormone-responsive as well as growth-related regulatory elements.11 differential expression genes were discovered in different developmental stages and various tissues,such as root,stem,leaf,flower and seed.

关 键 词：百脉根 生长素输出载体蛋白 PIN基因家族 生物信息学分析 

分 类 号：S567[农业科学—中草药栽培]