火龙果溃疡病原菌拮抗菌株的筛选与生物防治效果初探  

Screening of Antagonistic Strains of Pitaya Ulcer Pathogen and Primary Study on Biocontrol Effects

作　　者：张振华 林江 王文雅 许畅 杨开样[3] 何世伟 ZHANG Zhenhua;LIN Jiang;WANG Wenya;XU Chang;YANG Kaiyang;HE Shiwei(Qiongshan District Agriculture and Forestry Service Center of Haikou City,Haikou 571100,China;School of Tropic Agriculture and Forestry,Hainan University,Danzhou 571737,China;Experimental Field of Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,China)

机构地区：[1]海口市琼山区农林服务中心,海南海口571100 [2]海南大学热带农林学院,海南儋州571737 [3]中国热带农业科学院试验场,海南儋州571737

出　　处：《河南农业科学》2019年第8期88-94,共7页Journal of Henan Agricultural Sciences

基　　金：中国热带农业科学院基本科研业务费专项资金项目(1630092018012);海南省重点研发计划项目(ZDYF2018230);海南省自然科学基金项目(317084)

摘　　要：火龙果溃疡病为火龙果多发病害,为有效开展该病害的绿色防控,从火龙果不同生境进行了拮抗细菌的分离、筛选。从11个不同生境中共分离到103株细菌,应用对峙培养法进行了拮抗活性评价,筛选出15株具有拮抗活性的菌株,其中强拮抗性菌株5株,中等拮抗性菌株4株,弱拮抗性菌株6株。进一步对这些菌株进行生防物质分析,赋值≥10分的菌株有8株。综合以上结果,优选10个菌株进行温室防效测定,连续观察20d,7-6-1和10-4-6两个菌株的平均防效较高,分别为50.41%、48.85%。Pitaya ulcer is a frequently occurring disease for dragon fruits.To effectively carry out green prevention and control of this disease,antagonistic bacteria were isolated and screened from different habitats of dragon fruit.As a result,103 bacterial strains were isolated from 11 different habitats.Confrontation cultivation method was adopted to conduct antipathogenic activity evaluation,and 15 strains with antipathogenic activities were screened out,including 5 strains with strong antagonism,4 strains with medium antagonism and 6 strains with weak antagonism.Through further biocontrol active substance analysis of these strains,8 strains with values higher than 10 points were assigned.Based on the above results,10 strains were selected for the test of control effect in greenhouse and observed continuously for 20 days.The result showed that the strains 7-6-1 and 10-4-6 had higher control effects,which were 50.41% and 48.85% on average,respectively.

关 键 词：火龙果溃疡病 拮抗菌株 筛选 生物防治 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]