膜联蛋白A1及其模拟肽Ac2-26对高糖诱导大鼠心肌细胞炎症反应的影响  

Effect of Annexin A1 and Mimetic Peptide Ac2-26 on Inflammatory Response in Cardiac Myocytes Induced by High Glucose in Rats

作　　者：陈建康[1] 严瑜[1] 潘晓莉[1] 周密[1] 张文泉 杨洁 朱鹏立[1] CHEN Jiankang;YAN Yu;PAN Xiaoli;ZHOU Mi;ZHANG Wenquan;YANG Jie;ZHU Pengli(Department of Geriatric Medicine,Fujian Provincial Hospital,Provincial Clinical College,Fujian Medical University,Fujian Institute of Clinical Geriatrics,Fuzhou 350001,China)

机构地区：[1]福建省立医院老年科福建医科大学省立临床医学院福建省临床老年病研究所

出　　处：《中国医科大学学报》2019年第8期730-733,共4页Journal of China Medical University

基　　金：福建省自然科学基金（2018J01256）

摘　　要：目的探讨膜联蛋白A1及其模拟肽Ac2-26对高糖诱导大鼠心肌细胞炎症反应的影响。方法培养大鼠心肌细胞并随机分为正常对照组(葡萄糖5.5 mmol/L)、正常干预组[葡萄糖(5.5 mmol/L)+Ac2-26 (0.1 mg/mL)]、高糖模型组(葡萄糖30 mmol/L)、高糖治疗组[葡萄糖(30 mmol/L)+Ac2-26 (0.1 mg/mL)];采用实时PCR测定心肌细胞膜联蛋白A1、磷脂酶A2 (PLA2) mRNA表达,采用酶联免疫分析法测定心肌细胞肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)水平。结果与正常对照组比较,正常干预组膜联蛋白A1、PLA2 mRNA,TNF-α、IL-1β水平下降,但差异无统计学意义(P> 0.05)。与正常对照组比较,高糖模型组膜联蛋白A1、PLA2 mRNA表达,TNF-α、IL-1β水平显著升高(P <0.01)。与高糖模型组比较,高糖治疗组PLA2 mRNA表达,TNF-α、IL-1β水平明显下降(P <0.01);但膜联蛋白A1表达下降不显著(P> 0.05)。结论膜联蛋白A1与高糖诱导大鼠心肌细胞炎症反应明显相关,Ac2-26能够明显改善高糖诱导大鼠心肌细胞的炎症反应。Objective To study the effect of Annexin A1 and mimetic peptide Ac2-26 on myocardial inflammation induced by high glucose. Methods Rat cardiac myocytes were cultured and randomly divided into the following four groups:control(glucose 5.5 mmol/L),normal intervention [glucose(5.5 mmol/L)+Ac2-26(0.1 mg/mL)],high glucose(glucose 30 mmol/L),and high glucose treatment group[glucose(30 mmol/L)+Ac2-26(0.1 mg/mL)]. Expression of Annexin A1 and PLA2 mRNA in cardiac myocytes was measured by real-time PCR,and TNF-α and IL-1β level n was measured by enzyme-linked immunosorbent assay(ELISA). Results Annexin A1,PLA2 mRNA expression,TNF-α,and IL-1β level in the normal intervention group showed a decreasing trend compared to the normal control group.However,there was no significant difference(P > 0.05). Compared with the normal control group,Annexin A1,PLA2 mRNA expression,TNF-α,and IL-1β level increased significantly in the high glucose group(P < 0.01).Further,compared with the high glucose group,PLA2 mRNA expression,TNF-α,and IL-1β decreased significantly in the high glucose treatment group(P < 0.01),while Annexin A1 mRNA expression decreased slightly(P > 0.05). Conclusion Annexin A1 was significantly associated with high glucose-induced myocardial inflammation,and Ac2-26 significantly improved cardiomyocyte inflammation induced by high glucose inrat.

关 键 词：膜联蛋白A1 Ac2-26 心肌细胞 糖尿病 炎症反应 大鼠 

分 类 号：R544.1[医药卫生—心血管疾病]