慢性应激致抑郁小鼠不同组织C型钠尿肽表达的变化  

Changes of C-type natriuretic peptide expression in different tissues of mice with chronic stress-induced depression

作　　者：任敬一 付媛媛 荆潇橦 苏玉娇 史晓瑞 杜玉茹 史海水[2] REN Jingyi;FU Yuanyuan;JING Xiaotong;SU Yujiao;SHI Xiaorui;DU Yuru;SHI Haishui(School of Public Health,Hebei Medical University,Hebei Province,Shijiazhuang 050017,China;Center of Neuroscience Research,Institute of Medical and Health Science,Hebei Medical University,Hebei Province,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学公共卫生学院,河北石家庄050017 [2]河北医科大学医学与健康研究院神经科学研究中心,河北石家庄050017

出　　处：《中国医药导报》2019年第22期26-29,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81771462);河北省自然科学基金资助项目(H2018206119);河北医科大学大学生创新性实验项目(USIP2018069)

摘　　要：目的探讨慢性应激致抑郁小鼠不同组织和血清中C型钠尿肽(CNP)表达的影响。方法选取20只雄性ICR小鼠,并按照随机数字表法将其分为对照组和应激组,每组10只。采用慢性轻度应激(CMS)方法建立抑郁小鼠模型,经动物行为学检测后取材,采用酶联免疫吸附测定(ELISA)法对小鼠海马、肝、肾、心和血清中CNP进行定量分析。结果行为学测试结果显示,与对照组比较,应激组小鼠糖水偏爱值明显减小,强迫游泳测试、悬尾测试的不动潜伏期明显缩短,强迫游泳的不动时间明显延长,差异均有统计学意义(P<0.05);两组小鼠总摄水量比较,差异无统计学意义(P>0.05)。与对照组比较,应激组小鼠心、肾和血清中CNP水平明显升高,差异均有统计学意义(均P<0.05)。结论慢性应激可引起心、肾和血清中CNP水平显著升高,提示外周组织的CNP表达变化可能与应激致抑郁的发生发展有关。Objective To investigate the effects of C-type natriuretic peptide (CNP) expression in different tissues and serum of mice with chronic stress-induced depression.Methods Twenty male ICR mice were selected and divided into control group and stress group according to the random number table method with 10 mice in each group.Chronic mild stress (CMS) method was used to establish depression mice model.After animal behavior test,samples were taken and the levels of CNP in hippocampus,liver,kidney,heart and serum were quantitatively analyzed by enzyme-linked immunosorbent assay (ELISA).Results The results of behavioral test showed that compared with the control group,the preference value of sugar and water in stress group was significantly reduced,the immobility latency of forced swimming test and tail suspension test were significantly shortened,and the immobility time of forced swimming was significantly prolonged,the differences were statistically significant (P < 0.05).There was no significant difference in total water intake between the two groups (P > 0.05).Compared with the control group,the levels of CNP in the heart,kidney and serum of the stress group increased significantly (all P < 0.05).Conclusion Chronic stress can significantly increase the levels of CNP in heart,kidney and serum,suggesting that the changes of CNP expression in peripheral tissues may be related to the occurrence and development of stress-induced depression.

关 键 词：抑郁症 慢性应激 钠尿肽 C型钠尿肽 

分 类 号：R395[哲学宗教—心理学]