芥菜型油菜品种四川黄籽的BAC文库构建与分析  

Construction and characterization of BAC library of oilseed Brassica juncea variety Sichuan Yellow Seed

作　　者：罗松予 罗美中[2] 刘忠松[1] LUO Songyu;LUO Meizhong;LIU Zhongsong(Oil Crops Research Institute,Hunan Agricultural University,Changsha 410128,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]湖南农业大学油料作物研究所,长沙410128 [2]华中农业大学生命科学技术学院,武汉430070

出　　处：《华中农业大学学报》2019年第5期43-49,共7页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30971799)

摘　　要：为促进黄籽油菜的基因组研究和利用,构建了芥菜型油菜黄籽品种四川黄籽的高质量BAC文库。该文库由82944个单克隆组成,被保存在216块384孔板中。质粒酶切检测结果表明,文库的平均插入片段大小约为140kb,空载率约为2.5%,按照芥菜型油菜基因组1000Mb计算,文库覆盖度约为油菜基因组的11.6倍。BAC末端序列比对分析结果显示,克隆被均匀比对到芥菜型油菜的18条染色体上,并且无任何叶绿体、线粒体和细菌基因组DNA污染。A high-quality BAC library of the oilseed Brassica juncea variety Sichuan Yellow Seed was constructed to accelerate the study and utilization of the yellow-seed rapeseed genome.The library contains 82 944 clones and is stored in 216×384 microtiter plates.The results of restriction enzyme digestion of plasmid showed that the average insert size of the library was about 140 kb and the empty-vector rate was about 2.5%.Based on the Brassica juncea genome size of 1 000 Mb,the library covers about 11.6× genomes.The results of Blast analysis of the BAC end sequences showed that the clones were evenly mapped onto the 18 chromosomes of the Brassica juncea genome.No chloroplast,mitochondria and bacterium genome DNA contamination was found.

关 键 词：芥菜型油菜 黄籽 大片段DNA制备 BAC文库 质粒 酶切 

分 类 号：S565.4[农业科学—作物学]