SUMO特异肽酶3调控小鼠睾丸Sertoli细胞自噬  

SUMO specific peptidase 3 regulates autophagy in mouse testicular Sertoli cells

作　　者：陶亚群 朱慧琴 潘艺青[2] 劳一敏 易静[1] 杨洁[1] TAO Ya-qun;ZHU Hui-qin;PAN Yi-qing;LAO Yi-min;YI Jing;YANG Jie(Department of Biochemistry and Molecular Cell Biology,Shanghai Jiao Tong University College of Basic Medical Sciences,Shanghai 200025, China;Department of Histology, Embryology, Genetics and Developmental Biology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025,China)

机构地区：[1]上海交通大学基础医学院生物化学与分子细胞生物学系,上海200025 [2]上海交通大学基础医学院组织胚胎与遗传发育学系,上海200025

出　　处：《上海交通大学学报（医学版）》2019年第7期706-713,共8页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金面上项目（31771522）;上海市自然科学基金(16ZR1418400);2016上海慈善慢跑癌症研究基金(SCCRC16004)~~

摘　　要：目的·探讨小鼠睾丸中SUMO特异肽酶3(SUMO specific peptidase3,SENP3,又称SUMO特异蛋白酶3)对自噬的调控作用。方法·采用免疫荧光法对SENP3在睾丸生精细胞和支持细胞(Sertoli cells,简称Sertoli细胞)中的定位进行检测。对Senp3野生型(Senp3+/+)小鼠和Senp3基因敲除杂合子(Senp3+/-)小鼠进行饥饿处理,诱导自噬的发生。提取小鼠的睾丸组织蛋白质,采用Western blotting检测自噬的程度。利用透射电子显微镜技术、免疫荧光法检测睾丸组织切片中的细胞自噬,并区分睾丸生精细胞和Sertoli细胞自噬的程度。结果·SENP3主要定位于Sertoli细胞核。与Senp3+/+小鼠相比,Senp3+/-小鼠饥饿后,Sertoli细胞自噬增加。结论·SENP3能够抑制营养缺乏时Sertoli细胞自噬,可能对细胞自噬的程度发挥控制作用。Objective · To investigate the regulation of autophagy by SUMO specific peptidase 3 (SENP3, normally called SUMO specific protease 3) in mouse testis. Methods · Immunofluorescence was used to detect the localization of SENP3 in spermatogenic cells and Sertoli cells of testis. Senp3 wild type (Senp3+/+) mice and Senp3 gene knockout heterozygous (Senp3+/-) mice were subjected to starvation treatment to induce autophagy. Testicular tissue proteins were extracted, and the extent of autophagy was detected by Western blotting. The extent of autophagy of Sertoli cells was detected and compared with that of spermatogenic cells in testis by transmission electron microscopy and immunofluorescence. Results · SENP3 mainly localized in the nucleus of Sertoli cells. Compared to Senp3+/+ mice, the extent of starvation-induced autophagy in Sertoli cells of Senp3+/- mice increased. Conclusion · SENP3 can inhibit autophagy in Sertoli cells during nutrient deficiency, which may play a role in controlling the extent of autophagy.

关 键 词：自噬 SUMO特异蛋白酶3 睾丸 支持细胞(Sertoli细胞) 

分 类 号：Q291[生物学—细胞生物学]