新疆结核病患者外周血环状RNA差异表达分析  

Differential expression analysis of circRNA in peripheral blood of tuberculosis patients in Xinjiang

作　　者：孙畅 王家路[1] 刘艳 孟存仁[1] 张朝霞[1] SUN Chang;WANG Jialu;LIU Yan;MENG Cunren;ZHANG Zhaoxia(Department of Laboratory Medicine,the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang;Clinical Laboratory, Chest Hospital of Xinjiang Uyghur Autonomous Region, Urumqi 830049, Xinjiang, China)

机构地区：[1]新疆医科大学第一附属医院医学检验中心,乌鲁木齐830054 [2]新疆维吾尔自治区胸科医院检验科,乌鲁木齐830049

出　　处：《临床检验杂志》2019年第7期499-503,共5页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(81460323);新疆维吾尔自治区自然科学基金(2017D01C314)

摘　　要：目的了解新疆结核病患者全血环状RNA(circRNA)差异表达谱,并预测其靶基因,探索circRNA与结核病发生发展的关系。方法用表达谱芯片对肺结核患者和健康人对照各3例全血circRNA进行检测,筛选差异表达的circRNA;选择新疆43例肺结核患者、40例健康人对照者及43例肺炎患者全血进行qRT-PCR验证,用circRNA靶基因预测数据库进行差异circRNA靶microRNA的预测。结果芯片结果显示新疆结核病患者全血中存在差异表达的circRNA有835个,其中249个表达上调,586个表达下调。筛选出4个有显著差异的circRNA进行qRT-PCR验证,结果表明hsacirc0008276、hsacirc0003452、hsacirc0001846(P<0.05)和hsacirc0090508表达均下调(P<0.05),hsacirc0090508相较于其他3个circRNA特异性更高。circRNA靶基因预测结果提示,has-miR-1294、has-miR-604、has-miR-616、has-miR-663b、has-miR-486-3p可能是hsacirc0090508的潜在靶基因。结论 hsacirc0090508在结核病患者中的表达显著下调,其可能通过靶基因影响结核病的调控机制。Objective To find the differential expression profiles of circRNA in whole blood and predict its target genes in blood of patients with tuberculosis in Xinjiang, and explore the relationship between circRNA and the development of tuberculosis. Methods The circRNAs expression in peripheral blood from 3 pulmonary tuberculosis patients and 3 healthy individuals were tested by using circRNA microarray assay. The whole blood from 43 patients with tuberculosis, 40 healthy individuals and 43 patients with pneumonia were collected to verify the results by real-time quantitative PCR system. The possibility of differentially expressed circRNA target genes were predicted by circRNA target gene prediction database. Results In the results of microarray assay 835 circRNAs were found to be expressed differentially in whole blood between the tuberculosis patients and healthy controls of Xinjiang area, of which 249 circRNAs were up-regulated and 586 circRNAs were down-regulated in the patients. The expressions of four significantly different circRNA were verified by real time quantitative PCR and the results showed that hsacirc0008276, hsacirc0003452, hsacirc0001846 and hsacirc0090508 were down-regulated(P<0.05), and hsacirc0090508 was the more specific than the other three circRNAs. The results of circRNA target genes prediction suggested that has-miR-1294, has-miR-604, has-miR-616, has-miR-663 b and has-miR-486-3 p may be the potential target genes of hsacirc0090508. Conclusion The differentially expressed circRNA hsacirc0090508 was significantly downregulated in the patients with tuberculosis and may affect the regulation mechanism of tuberculosis through target genes.

关 键 词：结核病 环状RNA 基因芯片 靶基因 

分 类 号：R521[医药卫生—内科学] R446[医药卫生—临床医学]