微小RNA-133与微小RNA-208在急性心肌梗死患者中的免疫学作用  

The expression and immunological effect of miR-133 and miR-208 in acute myocardial infarction patients

作　　者：吴清柳[1] 李海涛[1] 任良强[1] 李海嵘[1] 周菲[1] WU Qingliu;LI Haitao;REN Liangqiang;LI Hairong;ZHOU Fei(Department of Vasculocardiology, People's Hospital of Hainan,Haikou 570311,China)

机构地区：[1]海南省人民医院心血管内科

出　　处：《免疫学杂志》2019年第8期704-710,共7页Immunological Journal

摘　　要：目的研究微小RNA-133与微小RNA-208在心肌梗死患者中的免疫学作用。方法 qPCR检测健康对照组与急性心肌梗死(acute myocardial infarction,AMI)组患者不同时段血浆中miR-133与miR-208的相对表达量;转染特异性干扰物与模拟物改变心肌HCM细胞中miR-133与miR-208的表达量,流式细胞仪检测HCM凋亡;CCK-8检测HCM细胞增殖活力;Western blot检测miR-133与miR-208对下游相关免疫调节蛋白CCL2、CX3CL1、RGC32的蛋白表达量的影响。结果 0 h与24 h组急性心肌梗死组患者中miR-133与miR-208较健康对照组均明显上升,7 d后恢复至正常水平;miR-133与miR-208可以促进心肌HCM细胞凋亡;miR-133与miR-208会减弱心肌HCM细胞的增殖活力;miR-133与miR-208会降低免疫调节蛋白CCL2、CX3CL1、RGC32的表达量。结论 AMI患者血浆miR-133与miR-208水平有显著升高,miR-133与miR-208可以通过介导急性心梗患者免疫调节因子CCL2、CX3CL1与RGC32的表达上调,促进心肌HCM细胞的增殖,抑制心肌HCM细胞的凋亡,从而起到保护心肌细胞的作用。This study was performed to investigate the immunological function of miR-133 and miR-208 increased in the acute myocardial infarction(AMI) patients, and to investigate the clinical relevance of miR-133 and miR-208 expression in AMI. The expression of miR-133 and miR-208 were detected by quantitative real time PCR(qRT-PCR) in blood of 138 AMI patients and 86 healthy controls;miRNA’s specific siRNA and mimics were transfected to change the levels of miR-133 and miR-208 in HCM cell. CCK-8 assays was used to detect the cell proliferation, FCM flow cytometry was employed to detect apoptosis rate, while Western blot was applied to measure the protein levels of CCL2/CX3 CL1/RGC32. Data showed that the levels of miR-133 and miR-208 in AMI group were higher than those of healthy group in 0 h and 24 h, and returned to normal levels 7 days later;miR-133 and miR-208 could promote HCM cell apoptosis and inhibit the HCM cell proliferation, and could up-regulate the protein of immune-regulation genes(CCL2/CX3 CL1/RGC32). In conclusion, miR-133 and miR-208 can promote the cell proliferation and inhibit the apoptosis of HCM by up-regulating the activities of CCL2/CX3 CL1/RGC32,thus protect the patients’ myocardial cells.

关 键 词：心肌梗死 miR-133 miR-208 免疫调节蛋白 

分 类 号：R542.2[医药卫生—心血管疾病]