秦皮乙素对S180小鼠肿瘤细胞膜功能的影响  

Effect of aesculetin on function of tumor cell membrane in S180 mice

作　　者：贾绍华 颜廷华 JIA Shao-hua;YAN Ting-hua(School of Honors,Harbin University of Commerce,Harbin 150018,China;School of Pharmacy,(Research Center of Pharmaceutical Engineering Technology),Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学英才学院,哈尔滨150018 [2]哈尔滨商业大学药学院(药物工程技术研究中心),哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2019年第3期265-268,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

摘　　要：研究秦皮乙素对S180小鼠肿瘤细胞膜功能的影响.采用抑瘤实验检测秦皮乙素对S180小鼠体内抗肿瘤活性;采用荧光光度法检测秦皮乙素对S180小鼠肿瘤细胞膜流动性的影响;采用比色法检测秦皮乙素对S180小鼠肿瘤细胞膜Na^+,K^+-ATPase和Ca^2+,Mg^2+-ATPase活性、含唾液酸量、封闭度的影响.秦皮乙素各组小鼠平均瘤重均低于阴性对照组,均可降低肿瘤细胞膜流动性、Na^+,K^+-ATPase和Ca^2+,Mg^2+-ATPase活性和膜封闭度,均可抑制肿瘤细胞膜唾液酸的生成.秦皮乙素抑制肿瘤细胞的生长与改变肿瘤细胞膜功能有关.To study the effect of aesculetin on the function of tumor cell membrane in S180 mice.Anti-tumor activity of aesculetin against S180 mice in vivo was detected by anti-tumor experiment;fluorescence spectrophotometry was used to detect the effect of aesculetin on the fluidity of tumor cell membrane in S180 mice;the effects of aesculetin on Na^+,K^+-ATPase and Ca^2+,Mg^2+-ATPase activity,sialic acid content and blocking degree of tumor cell membrane in S 180 mice were detected by colorimetry.Average tumor weight of all dosage groups for aesculetin was less than the negative control group,aesculetin can significantly reduce the activity of Na^+,K^+-ATPase and Ca^2+,Mg^2+-ATPase in tumor cell membrane of S180 mice,the membrane fluidity,the blocking degree and the content of sialic acid.The inhibition of the growth of tumor cells by aesculetin was related to the change of the function of tumor cell membrane.

关 键 词：秦皮乙素 S180小鼠 膜流动性 Na^+ K^+-ATPase Ca^2+ Mg^2+-ATPase 唾液酸 封闭度 

分 类 号：R284[医药卫生—中药学]