甘薯多糖SPP-Ⅰ的制备与结构初步研究  

Study on preparation and structure of sweet potato polysaccharide SPP-Ⅰ

作　　者：汲晨锋 张子依[1,2] 陈锦瑞 王昊 綦峥 于蕾[1,2] 刘日嘉 宋冬雪[1,2] 刘斌[1,2] JI Chen-feng;ZHANG Zi-yi;CHEN Jin-rui;WANG Hao;QI Zheng;YU Lei;LIU Ri-jia;SONG Dong-xue;LIU Bin(Engineering Research Center for Medicine,School of Pharmacy,Harbin University of Commerce,Harbin 150076,China;Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院药物工程技术研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2019年第3期306-312,共7页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省自然科学基金(H2015088);黑龙江省博士后特别资助项目(LBH-TZ0516);哈尔滨商业大学校级科研项目(18XN066)

摘　　要：研究采用水提醇沉法提取甘薯粗多糖,进行单因素试验考察,用正交法优化工艺.再经DEAE-纤维素-52阴离子交换柱和SephadexG200凝胶柱对所得粗多糖进行层析纯化,得到甘薯多糖组分SPP-Ⅰ.应用苯酚硫酸法测定多糖百分含量,HPLC法测定多糖分子质量,紫外光谱、红外光谱、甲基化衍生结合GC-MS分析多糖结构.结果表明甘薯多糖最优提取工艺为提取温度90℃,提取时间3h,液料比40∶1,提取次数3次.甘薯多糖SPP-Ⅰ总糖百分含量分别为97.61%,分子质量为245ku,紫外光谱扫描结果显示无核酸和蛋白质吸收峰,红外光谱扫描结果表明含有多糖类物质的特征吸收峰,结合甲基化和GC-MS分析初步确定甘薯多糖SPP-Ⅰ是具有1→3、1→3→6糖苷键结构的α-葡聚糖.Crude polysaccharides were extracted from sweet potato by hot water extraction and alcohol precipitation,single factor test and orthogonal method was carried out to optimize process.DEAE cellulose 52 and Sephadex G-200 gel filter column chromatographies were sequentially used to purify crude polysaccharides,and the purified polysaccharides SPP-Ⅰwere obtained.The content of the polysaccharide components of SPP-Ⅰwas determined by phenol sulfuric acid method,the molecular weight was analyzed by high performance liquid chromatography(HPLC),the structure was analyzed and characterized by ultraviolet(UV)spectroscopy,infrared(IR)spectroscopy and gas chromatograph mass(GC-MS).The results showed the optimized extration exprocess was as follows:extraction temperature at 90℃,extraction time for 3 h,liquid-solid ratio at 40∶1,extraction times for 3 times.And the total sugar content of SPP-Ⅰwas 97.61%,the molecular weights were 245 ku.The structural analysis demonstrated that SPP-Ⅰwas composed ofα-glucan with 1→3 and 1→3→6 linkage.

关 键 词：甘薯 多糖 制备 结构 

分 类 号：R284[医药卫生—中药学]