菲和芘胁迫下AMF和PGPR对高羊茅生理生态的响应  

Responses of AMF and PGPR to Festuca elata under phenanthrene and pyrene stress

作　　者：李文彬 宁楚涵 李伟 李峰 郭绍霞[1,2] LI Wen-bin;NING Chu-han;LI Wei;LI Feng;GUO Shao-xia(Institute of Mycorrhizal Biotechnology,Qingdao Agricultural University,Qingdao 266109,China;College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学菌根生物技术研究所,山东青岛266109 [2]青岛农业大学园林与林学院,山东青岛266109

出　　处：《草业学报》2019年第8期84-94,共11页Acta Prataculturae Sinica

基　　金：青岛市科技计划基础研究项目(121454)资助

摘　　要：旨在探究菲和芘胁迫下丛枝菌根真菌(AMF)与根围促生细菌(PGPR)对高羊茅生理生态的响应。以菲和芘1∶1混合处理浓度各0、50、100和150mg·kg^-1下对高羊茅接种AMF变形球囊霉(Gv)、PGPR荧光假单胞菌(Ps2-6)、Gv+Ps2-6和不接种对照共16个处理。结果表明,AMF、PGPR或AMF+PGPR处理均显著增加高羊茅生物量和菌根侵染率,增强植物光合作用,提高叶绿素含量,显著提高植物体内生理活性。在土壤中菲和芘100mg·kg^-1水平下,与对照相比,双接种Gv+Ps2-6处理的高羊茅叶片叶绿素a、b和总叶绿素含量分别提高57.7%、41.7%和51.8%;净光合速率(Pn)、蒸腾速率(Tr)、胞间CO2浓度(Ci)和气孔导度(Gs)分别增加70.6%、100.0%、4.5%和78.6%;最大光化学效率(Fv/Fm)和PSⅡ潜在活性(Fv/Fo)分别提高2.2%和8.8%;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、可溶性糖和脯氨酸含量分别是对照的1.6、1.5、2.3和2.7倍,丙二醛含量比对照下降46.0%;高羊茅株高和地上鲜重分别比对照增加63.0%和69.6%;接种PGPR处理能够增加AMF侵染率以及菌根依赖性。供试条件下,双接种Gv+Ps2-6处理增加高羊茅叶绿素含量和抗氧化能力,增强光合作用能力,降低膜脂过氧化水平,促进植物生长的作用最为显著。The purpose of the present study was to evaluate mechanisms(and their efficiency)for degradation of residues of phenanthrene and pyrene by arbuscular mycorrhizal fungi(AMF)and plant growth-promoting rhizobacteria(PGPR).A total of 16 treatments were designed involving Festuca elata,with or without inoculation with AMF Glomus versiforme(Gv),PGPR Pseudomonas fluorescens(Ps2-6),or Gv+Ps2-6,and grown soil with 0(Control),50,100 or 150 mg·kg^-1 added polycyclic aromatic hydrocarbons(PAHs)(phenanthrene∶pyrene=1∶1 in weight).The results showed that inoculation with AMF,PGPR or AMF+PGPR significantly increased the biomass and mycorrhizal infection rate of tall fescue,enhanced photosynthesis and chlorophyll content of plants,and significantly increased the physiological activity of plants.At 100 mg·kg^-1 phenanthrene and pyrene,the chlorophyll a,b and total chlorophyll content in the leaves of tall fescue were increased by 57.7%,41.7%and 51.8%,respectively,compared with the Control,and the net photosynthetic rate(P n),transpiration rate(T r),intercellular CO 2 concentration(C i)and gas pore conductivity(G s)were increased by 70.6%,100.0%,4.5%and 78.6%,respectively;the maximum photochemical efficiency(F v/F m)and PSⅡpotential activity(F v/F o)were increased by 2.2%and 8.8%,respectively.The activities of superoxide dismutase(SOD),catalase(CAT),soluble sugar and proline were,respectively,1.6,1.5,2.3 and 2.7 times those of the Control;the malondialdehyde content was decreased by 46.0%,and the height and fresh weight were increased by 63.0%and 69.6%.Inoculation with PGPR increased AMF infection rate and mycorrhizal dependency.Under the test conditions,double inoculation with a Gv+Ps2-6 treatment increased the content of photosynthetic pigments and antioxidant capacity of Festuca elata,enhanced photosynthetic capacity,reduced the level of membrane lipid peroxidation,and significantly promoted plant growth.

关 键 词：丛枝菌根真菌 根围促生细菌 菲和芘 光合 叶绿素 生理 

分 类 号：S688.4[农业科学—观赏园艺] X592[农业科学—园艺学]