病原体核酸技术在儿童感染性疾病检测中的发展及应用  

The development and application of nucleic acid detection techniques of infectious microorganisms in pediatrics

作　　者：李伟[1] 陶然[1] 尚世强[1] Li Wei;Tao Ran;Shang Shiqiang(Laboratory Center, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China)

机构地区：[1]浙江大学医学院附属儿童医院实验检验中心,杭州310052

出　　处：《中华检验医学杂志》2019年第7期489-492,共4页Chinese Journal of Laboratory Medicine

摘　　要：分子诊断技术的发展加速推进了儿童感染性疾病病原体核酸检测向高通量、高灵敏度、自动化发展的进程。在临床常用的荧光定量PCR技术之外,多重PCR技术、数字PCR技术、等温扩增技术、微流控芯片的应用使病原体核酸检测在通量和灵敏度上有了显著提升,这些技术配合一体机的使用实现了儿童感染性疾病病原体核酸检测的自动化和高通量化。高通量测序技术可以最大程度获取标本中几乎所有病原体的核酸信息,已逐渐成为临床感染性疾病诊断的重要辅助手段。The advance of molecular diagnosis has evolved the field of detection of pathogens in pediatric disease towards the high-throughput ultra-sensitive molecular testing. This review provides an overview and comparison of current molecular tools on the market, including qPCR, multiplex PCR, digital PCR, isothermal amplification, and microfluidic chip, highlighting the future trends of integrated high-throughput molecular testing and automation. These evolutions will maximize the utility of limit patient samples from children and contribute significantly on the diagnosis of infectious disease.

关 键 词：传染病 高通量核苷酸序列分析 儿童 

分 类 号：R725.1[医药卫生—儿科] R440[医药卫生—临床医学]