拉曼光谱技术在男性生殖中的应用  

The application of Raman spectroscopy in male reproduction diagnosis

作　　者：刘雪丽 常椿欣 徐晨明[1] Liu Xueli;Chang Chunxin;Xu Chenming(Key Laboratory of Reproductive Genetics, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China)

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院生殖遗传科,上海200030

出　　处：《中华检验医学杂志》2019年第7期498-502,共5页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金(81771638).

摘　　要：目前临床上男性不育患者的诊治手段有限,随着辅助生殖技术的不断发展,对精度更高的技术的需求日益迫切。拉曼光谱技术是一种高灵敏度的、样品无标记无损的快速分析技术,可以检测到分子层面的异常,是评估男性生育力和协助辅助生殖治疗的重要方法。本文综述了拉曼光谱技术的基本原理、主要分类及其在精液分析等男性生殖领域中的研究近况。The limited method demands for new technology in the management of male infertility. Raman spectroscopy is a rapid and sensitive non-invasive diagnosing technology. Raman spectroscopy was able to obtain Raman spectra from individual human sperm cells and allows for the comparison of their biochemical components, DNA and mitochondrial structure as well as epigenetic changes. This review provided an overview of the principle of each major types and discussed the progress in clinical application, indicating the potential of Raman spectroscopy as an valuable addition in assessing the quality of sperm cells for in vitro fertilization.

关 键 词：不育 男(雄)性 精子 精液 光谱分析 拉曼 

分 类 号：O657.37[理学—分析化学] R698.2[理学—化学]