慢性阻塞性肺疾病伴肺部感染者相关细胞因子的表达及其与肺功能的关系  

Correlation between expression of cytokines and lung function of chronic obstructive pulmonary disease patients complicated with pulmonary infection

作　　者：张晶[1] 栾晓嵘[2] 郝俊萍[1] 张长敏[1] 李红梅[2] ZHANG Jing;LUAN Xiao-rong;HAO Jun-ping;ZHANG Chang-min;LI Hong-mei(Qilu Hospital of Shandong University Jinan , Shandong 250012, China)

机构地区：[1]山东大学齐鲁医院呼吸与危重症医学科,山东济南250012 [2]山东大学齐鲁医院护理部,山东济南250012

出　　处：《中华医院感染学杂志》2019年第13期1954-1958,共5页Chinese Journal of Nosocomiology

基　　金：山东省卫生厅重点科技基金资助项目(2014WTS1536)

摘　　要：目的探讨慢性阻塞性肺疾病(COPD)伴肺部感染患者炎症因子、辅助性T细胞17(Th17)和调节性T细胞(Treg)的表达及其临床意义。方法将COPD伴肺部感染者70例为合并感染组,单纯COPD者70例为COPD组,以及健康志愿者70例为对照组。比较三组白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(INF-γ)、Th17、Treg的表达情况,并分析与肺功能的关系。结果合并感染组IL-6、TNF-α、INF-γ高于COPD组和对照组,COPD组则高于对照组(P<0.05),合并感染组Th17细胞比例高于COPD组和对照组,Treg细胞比例低于COPD组和对照组(P<0.05),COPD组Treg细胞比例低于对照组(P<0.05),合并感染组的用力肺活量(FVC)、一秒钟用力呼气量(FEV1)、FEV1/FVC低于COPD组和对照组,COPD组的FVC、FEV1、FEV1/FVC低于对照组(P<0.05),COPD并发肺部感染患者IL-6、TNF-α、INF-γ、Th17细胞比例与肺功能指标FVC、FEV1、FEV1/FVC均呈负相关(P<0.05),Treg细胞比例与肺功能指标FVC、FEV1、FEV1/FVC均呈正相关(P<0.05)。结论在COPD并发肺部感染患者中炎性因子和Th17呈高表达,Treg呈低表达,炎症因子和Th17、Treg介导的炎症反应可影响患者的肺功能。OBJECTIVE To explore the expression of inflammatory factors, type 17 helper T cells(Th17) and regulatory T(Treg) cells in the chronic obstructive pulmonary disease(COPD) patients complicated with pulmonary infection. METHODS Totally 70 COPD patients who were complicated with pulmonary infection were assigned as the complicated infection group, 70 patients who had single CODP were assigned as the COPD group, and 70 healthy volunteers were set as the control group. The levels of expression of interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), interferon-γ(INF-γ), Th17 and Treg were observed and compared among the three groups, and their association with lung function was analyzed. RESULTS The levels of IL-6, TNF-α and INF-γ of the complicated infection group were sighificantly higher than those of the COPD group and the control group, and the levels of the above indexes of the COPD group were sighificantly higher than those of the control group(P<0.05);the percentage of Th cells of the complicated infection group was sighificantly higher than that of the COPD group and the control group;the percentage of Treg cells of the complicated infection group was sighificantly lower than that of the COPD group and the control group(P<0.05), and the percentage of Treg cells of the COPD group was lower than that of the control group. The forced vital capacity(FVC), forced expiratory volume in 1 second(FEV1) and FEV1/FVC were sighificantly lower in the complicated infection group than in the COPD group and the control group, and the FVC, FEV1 and FEV1/FVC were sighificantly lower in the COPD group than in the control group(P<0.05). The levels of IL-6, TNF-α and INF-γ and percentage of Th17 cells of the COPD patients complicated with pulmonary infection were negatively correlated with the lung function indexes FVC, FEV1 and FEV1/FVC(P<0.05);the percentage of Treg cells was positively correlated with the lung function indexes FVC, FEV1 and FEV1/FVC(P<0.05). CONCLUSION The levels of inflammatory factors and percentage of

关 键 词：慢性阻塞性肺疾病 肺部感染 炎症因子 辅助性T细胞17 调节性T细胞 肺功能 

分 类 号：R563.3[医药卫生—呼吸系统]