缺氧MBBR耦合部分厌氧氨氧化强化城市生活污水深度脱氮  

Advanced Denitrification of Municipal Wastewater Achieved via Partial ANAMMOX in Anoxic MBBR

作　　者：杨岚 彭永臻[1] 李健伟[1] 高锐涛 王茗 李夕耀[1] YANG Lan;PENG Yong-zhen;LI Jian-wei;GAO Rui-tao;WANG Ming;LI Xi-yao(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China)

机构地区：[1]北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室北京市水质科学与水环境恢复工程重点实验室

出　　处：《环境科学》2019年第8期3668-3674,共7页Environmental Science

基　　金：北京市科技计划项目(D171100001017001);北京市教委项目

摘　　要：缺氧MBBR是强化传统城市污水处理系统脱氮的一种方法,本研究通过向城市污水后置反硝化SBR中投加填料构建了缺氧双污泥系统,实现了城市生活污水部分厌氧氨氧化深度脱氮.在250d的运行中脱氮性能逐渐提高并实现稳定,出水总氮在5 mg·L^-1左右. 211~250 d的平均硝氮、氨氮和总氮去除率分别为(97. 7±2. 9)%、(93. 3±2. 9)%和(94. 3±2. 7)%.长期运行中观测到氨氮和硝氮的同步去除.针对氨氮去除途径进行分析,系统同化、硝化作用微弱.缺氧MBBR中存在厌氧氨氧化活性且对脱氮有不可忽视的作用.实时定量PCR结果进一步说明缺氧MBBR中厌氧氨氧化菌富集,特别是缺氧填料生物膜中厌氧氨氧化菌丰度由初始的4. 37×10^7copies·g^-1增长到了2. 28×1010copies·g^-1.本研究表明缺氧填料生物膜在厌氧氨氧化的富集强化城市污水深度脱氮中或许具有可应用的潜能.Anoxic MBBR is a process to achieve advanced denitrification from municipal wastewater. Here,anoxic MBBR was applied as a post-denitrification SBR to achieve advanced denitrification by partial anammox( anaerobic ammonium oxidation). During a 250-day operation,denitrification performance gradually improved and the total nitrogen concentration of the effluent was approximately 5 mg·L^-1. The average nitrate,ammonia,and total inorganic nitrogen removal efficiencies were( 97. 7 ± 2. 9)%,( 93. 3 ± 2. 9)%,and( 94. 3 ± 2. 7)%,respectively,between day 211 and 250. The simultaneous removal of ammonia and nitrate was observed in the anoxic reactor. Analysis of the ammonia removal pathway revealed that assimilation and nitrification were poor in the anoxic MBBR.The anammox activity test and the denitrification performance showed that anammox occurred and played a not insignificant role in the anoxic MBBR. The results of real-time quantitative PCR showed that anammox bacteria enriched in anoxic MBBR,especially in the anoxic carrier biofilms,where the abundance of anammox bacteria increased from 4. 37 × 10^7 copies·g^-1 to 2. 28 × 1010 copies·g^-1.This study demonstrates that anoxic carrier biofilms may have potential applications in anammox bacterial enrichment to enhance denitrification from municipal wastewater.

关 键 词：深度脱氮 厌氧氨氧化(ANAMMOX) 缺氧生物膜 城市生活污水 生物膜移动床反应器(MBBR) 

分 类 号：X703.[环境科学与工程—环境工程]