慢性间歇低氧对大鼠下丘脑-垂体-生长激素轴的影响  

The influence of chronic intermittent hypoxia on hypothalamic-somatotropic axis in rats

作　　者：徐家欢[1] 申慧[1] 李文扬[1] 孟艳玲 王玮[1] Xu Jiahuan;Shen Hui;Li Wenyang;Meng Yanling;Wang Wei(Department of Respiratory and Critical Care Medicine, The First Hospital of China Medical University,Shenyang 110001, China)

机构地区：[1]中国医科大学附属第一医院呼吸与危重症医学科,沈阳110001

出　　处：《中华内科杂志》2019年第8期592-595,共4页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金(81670085);辽宁省特聘教授(辽教发[2015]153号).

摘　　要：目的探讨慢性间歇低氧对大鼠下丘脑-垂体-生长激素轴激素水平的影响。方法将雄性Wistar大鼠分为常氧组和慢性间歇低氧组,将慢性间歇低氧组大鼠置于低氧舱内,每天8 h,每180秒向舱内交替输送21%氧气(空气)和5%氧气(低氧气体),采用数字测氧仪监测舱内氧气含量,调节气体流量,持续12周。常氧组大鼠置于同样环境下,但给予21%氧气(空气)暴露。分别于气体暴露前、暴露第4周、第8周、第12周时检测2组大鼠血清生长激素释放激素、生长激素、生长抑素水平。结果与常氧组比,慢性间歇低氧组大鼠在低氧第4周即出现生长激素释放激素水平下降[(732.77±46.99)pg/ml比(893.59±40.00)pg/ml],第8周出现生长抑素水平下降[(30.71±2.27)pg/ml比(44.69±3.36)pg/ml],第12周才出现生长激素水平下降[(1.20±0.29)ng/ml比(2.06±0.13)ng/ml],差异均有统计学意义(P值均<0.05)。随着间歇低氧时间的延长,生长激素释放激素水平未进一步下降[4周为(732.77±46.99)pg/ml,8周为(607.64±131.61)pg/m,12周为(730.05±40.63)pg/m,P>0.05],但血清生长抑素水平从第8周至第12周出现了进一步降低[8周为(30.71±2.27)pg/ml,12周为(24.41±4.06)pg/ml,P<0.05]。结论慢性间歇低氧可能抑制下丘脑-垂体-生长激素轴功能,下丘脑激素最先受到影响,后期整个激素轴受累。Objective To investigate the effects of chronic intermittent hypoxia on somatotropic axis hormone levels in rats. Methods Mature male Wistar rats were exposed to air or intermittent hypoxia randomly.The serum levels of growth hormone-releasing hormone (GHRH), growth hormone (GH) and somatostatin (SS) were measured before exposure, at the 4th, 8th, and 12th week after exposure. Different hormone levels in two groups were compared and analyzed. Results Compared with the control group, GHRH levels in chronic intermittent hypoxic group showed a significant decline at the 4th week [(732.77±46.99)pg/ml vs.(893.59±40.00) pg/ml, P<0.05], while SS levels at the 8th week [(30.71±2.27) pg/ml vs.(44.69±3.36) pg/ml, P<0.05] and GH levels at the 12th week [(1.20±0.29) ng/ml vs.(2.06±0.13) ng/ml, P<0.05] were similarly reduced. As the duration of intermittent hypoxia was prolonged, the GHRH levels did not decrease further [4th week (732.77±46.99) pg/ml vs. 8th week (607.54±131.61) pg/ml vs. 12th week (730.05±40.63) pg/ml, P>0.05].However, the serum SS levels decreased further from the 8th week to the 12th week [(30.71±2.27) pg/ml vs.(24.41±4.06) pg/ml, P<0.05]. Conclusion Chronic intermittent hypoxia might inhibit the function of somatotropic axis. Hypothalamic hormones are the earlyonesto be influenced, thereafter the entire axis.

关 键 词：慢性间歇低氧 生长激素释放激素 生长激素 生长抑素 

分 类 号：R766[医药卫生—耳鼻咽喉科]