基于气道炎症调控的全真一气汤干预肾气虚型哮喘大鼠的机制研究  

Mechanisms of Quanzhen Yiqi Decoction on Bronchial Asthma Rats with Kidney-Qi Deficiency Based on Regulation of Airway Inflammation

作　　者：张晶 陈志斌 王春娥 李大治 Zhang Jing;Chen Zhibin;Wang Chun′e(The Second People′s Hospital Affiliated to Fujian University of Chinese Medicine,Fujian,Fuzhou 350003,China.)

机构地区：[1]福建中医药大学附属第二人民医院

出　　处：《中国中医急症》2019年第8期1354-1357,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：福建省自然科学基金项目(2016J01564)

摘　　要：目的观察全真一气汤对肾气虚型哮喘大鼠的干预作用,并基于气道炎症探讨其可能机制。方法 39只雄性清洁级SD大鼠随机分为5组:对照组7只,制备肾气虚型哮喘大鼠模型,随机分为模型组、低剂量组、中剂量组、高剂量组,每组各8只。采用ELISA法测定大鼠血清白细胞介素-4(IL-4)、肿瘤坏子因子-α(TNF-α)、水通道蛋白5(AQP5)水平,采用Western blotting法测定肺组织MUC5AC mRNA、OX-62 mRNA、AQP5 mRNA表达水平。结果肾气虚型哮喘大鼠血清IL-4、TNF-α,肺组织MUC5AC mRNA、OX-62 mRNA的表达水平均较对照组明显升高(P <0.01),给予药物作用后,其表达水平下调,且随药物剂量增加下调明显(P <0.01);相对于对照组,肾气虚型哮喘大鼠血清AQP5、肺组织AQP5 mRNA表达水平明显降低,给予药物作用后,其表达水平增加,且随药物剂量增加而增加明显(P <0.01)。结论全真一气汤可以改善肾气虚型哮喘大鼠的气道炎症,其机制可能与上调血清AQP5、肺组织AQP5 mRNA的表达水平有关。Objective:To observe the intervention effect of Quanzhen Yiqi Decoction on bronchial asthma ratswith kidney-Qi deficiency and discuss its possible mechanism based on airway inflammation. Methods:7 from 39 male clean SD rats was the control group;the rest were made into bronchial asthma rat models with kidney-Qideficiency and randomly divided into 4 groups:the model group,the low dose group,the medium dose group andthe high dose group(n = 8). The expression of IL-4,TNF-α and AQP5 in serum was detected by ELISA. ThemRNA expressions of MUC5AC,OX-62 and AQP5 in lung tissue were detected by Western blotting. Results:The mRNA expression of IL-4 and TNF-α in rat serum and MUC5 AC and OX-62 in lung tissue was significantlyincreased compared with that in the control group(P < 0.01). After drug intervention,the expression was decreasedwith the dosage(P < 0.01). The expression of AQP5 in rat serum and its mRNA expression in lung tissue were de-creased significantly compared with those in the model group. After drug intervention,the expression was in-creased with the dosag(P < 0.01). Conclusion:Quanzhen Yiqi Decoction has a protective effect on airway inflam-mation in rats with bronchial asthma of kidney Qi deficiency. Its possible mechanism is up-regulating AQP5 in ratserum and its mRNA expression in lung tissue.

关 键 词：哮喘 气道炎症 全真一气汤 肾气虚 机制 大鼠 

分 类 号：R285.5[医药卫生—中药学]