水势和盐胁迫对拟南芥种子萌发和根部生长的影响  

Effects of Water Potential and Salt Stress on Seed Germination and Root Growth in Arabidopsis

作　　者：高润昕 黄凤珍 李萌[1,2] 彭继庆 董旭杰[1,2] 曹福祥 GAO Runxin;HUANG Fengzhen;LI Meng;PENG Jiqing;DONG Xujie;CAO Fuxiang(College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410018;Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Changsha, Hunan 410018;Horticulture & Landscape College, Hunan Agricultural University, Changsha, Hunan 410004)

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410018 [2]湖南省环境资源植物开发与利用工程技术研究中心,湖南长沙410018 [3]湖南农业大学园艺园林学院,湖南长沙410004

出　　处：《北方园艺》2019年第15期8-14,共7页Northern Horticulture

基　　金：湖南省重点研发计划资助项目(2016NK2143);湖南省重点研发计划资助项目(2017NK2252)

摘　　要：以拟南芥为试验材料,向培养基中添加不同浓度的NaCl模拟盐胁迫、不同比例的PEG-6000模拟水势胁迫,观察并记录种子萌发率、幼苗根长、幼苗存活率等生理指标,检测幼苗中丙二醛(MDA)含量,研究了盐胁迫和水势胁迫对拟南芥种子萌发及幼苗生长的影响,以期明确盐胁迫程度和水势影响拟南芥植株生理状态的规律,为水势和盐胁迫处理提供依据。结果表明:当NaCl浓度等于及高于150 mmol·L-1时,幼苗平均根长为5.2 mm,显著低于对照组(24.8 mm);幼苗平均存活率为52.1%,显著低于对照组(98.1%)。当水势等于及低于-0.7 MPa时,幼苗无侧根生长,平均存活率为89.2%,显著低于对照组(98.7%),由此可知,NaCl浓度150 mmol·L-1和水势-0.7 MPa是拟南芥胁迫处理的阈值,当NaCl浓度高于150 mmol·L-1,或水势低于-0.7 MPa时,拟南芥种子萌发及幼苗生长被明显抑制。To clarify the effects of salt stress and water potential on seed germination and seedling growth in Arabidopsis. NaCl and PEG-6000 with different concentration was added into medium,respectively,to simulate salt stress and water potential of different degree.The effects on seed germination rate,root length,survival rate of seedling and MDA content in seedling were measured.The results showed that while the concentration of NaCl solution was equal to or higher than 150 mmol·L-1 the average root length of treated group was 5.2 mm,which was significantly less than that(24.8 mm) in the control group.The average seedling survival rate was 52.1%,which was significantly less than that(98.1%) in the control group.When the water potential was less than-0.7 MPa,the average seedling survival rate was 89.2%,which was significantly less than that(98.7%) in the control group.Moreover,there was no lateral root in Arabidopsis seedlings growing under-0.7 MPa of water potential.In conclusion,the 150 mmol·L-1 concentration of NaCl and-0.7 MPa were the thresholds for salt stress and water potential,respectively,to cause inhibiting effects on seed germination and seedling growth in Arabidopsis.

关 键 词：拟南芥 盐胁迫 水势 

分 类 号：Q945.65[生物学—植物学]